Recombinant Human PDX1 protein (ab114175)

Overview

  • Product nameRecombinant Human PDX1 protein
  • Protein lengthFull length protein

Description

  • NatureRecombinant
  • SourceWheat germ
  • Amino Acid Sequence
    • AccessionP52945
    • SpeciesHuman
    • SequenceMNGEEQYYAATQLYKDPCAFQRGPAPEFSASPPACLYMGRQPPPPPPHPF PGALGALEQGSPPDISPYEVPPLADDPAVAHLHHHLPAQLALPHPPAGPF PEGAEPGVLEEPNRVQLPFPWMKSTKAHAWKGQWAGGAYAAEPEENKRTR TAYTRAQLLELEKEFLFNKYISRPRRVELAVMLNLTERHIKIWFQNRRMK WKKEEDKKRGGGTAVGGGGVAEPEQDCAVTSGEELLALPPPPPPGGAVPP AAPVAAREGRLPPGLSASPQPSSVAPRRPQEPR
    • Molecular weight58 kDa including tags
    • Amino acids1 to 283

Specifications

Our Abpromise guarantee covers the use of ab114175 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    SDS-PAGE

    Western blot

    ELISA

  • FormLiquid
  • Additional notesProtein concentration is above or equal to 0.05 mg/ml.
    Best used within three months from the date of receipt.
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    pH: 8.00
    Constituents: 0.79% Tris HCl, 0.3% Glutathione

General Info

  • Alternative names
    • Glucose sensitive factor
    • Glucose-sensitive factor
    • GSF
    • IDX 1
    • IDX-1
    • IDX1
    • Insulin promoter factor 1
    • Insulin promoter factor 1 homeodomain transcription factor
    • Insulin upstream factor 1
    • IPF 1
    • IPF-1
    • IPF1
    • Islet/duodenum homeobox 1
    • Islet/duodenum homeobox-1
    • IUF 1
    • IUF-1
    • IUF1
    • MODY4
    • Pancreas/duodenum homeobox 1
    • Pancreas/duodenum homeobox protein 1
    • pancreatic and duodenal homeobox P
    • PDX 1
    • PDX-1
    • PDX1
    • PDX1_HUMAN
    • Somatostatin transactivating factor 1
    • Somatostatin-transactivating factor 1
    • STF 1
    • STF-1
    • STF1
    see all
  • FunctionActivates insulin, somatostatin, glucokinase, islet amyloid polypeptide and glucose transporter type 2 gene transcription. Particularly involved in glucose-dependent regulation of insulin gene transcription. Binds preferentially the DNA motif 5'-[CT]TAAT[TG]-3'. During development, specifies the early pancreatic epithelium, permitting its proliferation, branching and subsequent differentiation. At adult stage, required for maintaining the hormone-producing phenotype of the beta-cell.
  • Tissue specificityDuodenum and pancreas (Langerhans islet beta cells and small subsets of endocrine non-beta-cells, at low levels in acinar cells).
  • Involvement in diseaseDefects in PDX1 are a cause of pancreatic agenesis (PAC) [MIM:260370]. This autosomal recessive disorder is characterized by absence or hypoplasia of pancreas, leading to early-onset insulin-dependent diabetes mellitus. This was found in a frameshift mutation that produces a truncated protein and results in a second initiation that produces a second protein that act as a dominant negative mutant.
    Defects in PDX1 are a cause of non-insulin-dependent diabetes mellitus (NIDDM) [MIM:125853]; also known as diabetes mellitus type 2. NIDDM is characterized by an autosomal dominant mode of inheritance, onset during adulthood and insulin resistance.
    Defects in PDX1 are the cause of maturity-onset diabetes of the young type 4 (MODY4) [MIM:606392]; also symbolized MODY-4. MODY is a form of diabetes that is characterized by an autosomal dominant mode of inheritance, onset in childhood or early adulthood (usually before 25 years of age), a primary defect in insulin secretion and frequent insulin-independence at the beginning of the disease.
  • Sequence similaritiesBelongs to the Antp homeobox family. IPF1/XlHbox-8 subfamily.
    Contains 1 homeobox DNA-binding domain.
  • DomainThe Antp-type hexapeptide mediates heterodimerization with PBX on a regulatory element of the somatostatin promoter.
    The homeodomain, which contains the nuclear localization signal, not only mediates DNA-binding, but also acts as a protein-protein interaction domain for TCF3(E47), NEUROD1 and HMG-I(Y).
  • Post-translational
    modifications
    Phosphorylated by the SAPK2 pathway at high intracellular glucose concentration.
  • Cellular localizationNucleus.
  • Information by UniProt

Recombinant Human PDX1 protein images

  • ab114175 on a 12.5% SDS-PAGE Stained with Coomassie Blue.

References for Recombinant Human PDX1 protein (ab114175)

ab114175 has not yet been referenced specifically in any publications.

Product Wall

Yes, you can submit an Abreview for EMSA. Please select "Other" in the application field, and also please submit as much information as you are able to provide.

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