The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
% SDS-PAGE. ab123832 is purified by proprietary chromatographic techniques.
Additional notesStorage: Store lyophilized protein at -20°C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles. Reconstituted protein can be stored at 4°C for a limited period of time, but remains stable until the expiry date when stored at -20°C.
ReconstitutionAdd deionized water to a working concentration of 0.5 mg/ml and let the lyophilized pellet dissolve completely.
Cell proliferation-inducing gene 35 protein
Pigment epithelium derived factor
Pigment epithelium-derived factor
Proliferation inducing protein 35
serine (or cysteine) proteinase inhibitor, clade F (alpha-2 antiplasmin, pigment epithelium derived factor), member 1
Serpin family F member 1
Serpin peptidase inhibitor clade F member 1
serpin peptidase inhibitor, clade F (alpha-2 antiplasmin, pigment epithelium derived factor), member 1
FunctionNeurotrophic protein; induces extensive neuronal differentiation in retinoblastoma cells. Potent inhibitor of angiogenesis. As it does not undergo the S (stressed) to R (relaxed) conformational transition characteristic of active serpins, it exhibits no serine protease inhibitory activity.
Tissue specificityRetinal pigment epithelial cells and blood plasma.
Sequence similaritiesBelongs to the serpin family.
Developmental stageExpressed in quiescent cells.
DomainThe N-terminal (AA 44-121) exhibits neurite outgrowth-inducing activity. The C-terminal exposed loop (AA 382-418) is essential for serpin activity.
Post-translational modificationsThe N-terminus is blocked. Extracellular phosphorylation enhances antiangiogenic activity. N- and O-glycosylated. O-glycosylated with a core 1 or possibly core 8 glycan.
Cellular localizationSecreted. Melanosome. Enriched in stage I melanosomes.