Recombinant Human Peptidase inhibitor 16 protein (ab117229)

Overview

Description

  • Nature
    Recombinant
  • Source
    HEK 293 cells
  • Amino Acid Sequence
    • Accession
    • Species
      Human
    • Sequence
      LTDEEKRLMV ELHNLYRAQV SPPASDMLHM RWDEELAAFA KAYARQCVWG HNKERGRRGE NLFAITDEGM DVPLAMEEWH HEREHYNLSA ATCSPGQMCG HYTQVVWAKT ERIGCGSHFC EKLQGVEETN IELLVCNYEP PGNVKGKRPY QEGTPCSQCP SGYHCKNSLC EPIGSPEDAQ DLPYLVTEAP SFRATEASDS RKMGTPSSLA TGIPAFLVTE VSGSLATKAL PAVETQAPTS LATKDPPSMA TEAPPCVTTE VPSILAAHSL PSLDEEPVTF PKSTHVPIPK SADKVTDKTK VPSRSPENSL DPKMSLTGAR ELLPHAQEEA EAEAELPPSS EVLASVFPAQ DKPGELQATL DHTGHTSSKS LPNFPNTSAT ANATGGRALA LQSSLPGAEG PDKPSVVSGL NSGPGAAADY KDDDDK
    • Molecular weight
      46 kDa including tags
    • Amino acids
      28 to 442
    • Tags
      DDDDK tag C-Terminus

Specifications

Our Abpromise guarantee covers the use of ab117229 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Western blot

    ELISA

  • Endotoxin level
    < 1.000 Eu/µg
  • Form
    Lyophilised
  • Additional notes
    Store lyophilized protein at -80°C. Lyophilized protein remains stable until the expiry date when stored at -80°C. Aliquot reconstituted protein to avoid repeated freezing/thawing cycles and store at -80°C for long term storage. Reconstituted protein can be stored at 4°C for a week.
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at -80°C.

    pH: 7.50
    Constituents: 0.32% Tris HCl, 0.3% Sodium chloride

  • Reconstitution
    Add deionized water to prepare a working stock solution of 0.5 mg/mL and let the lyophilized pellet dissolve completely. Product is not sterile! Please filter the product by an appropriate sterile filter before using it in the cell culture.

General Info

  • Alternative names
    • Cripi
    • CRISP 9
    • CRISP-9
    • CRISP9
    • Cysteine rich protease inhibitor
    • Cysteine rich secretory protein 9
    • Cysteine-rich secretory protein 9
    • Microseminoprotein beta binding protein
    • MSMBBP
    • Peptidase inhibitor 16
    • PI-16
    • PI16
    • PI16_HUMAN
    • Protease inhibitor 16
    • PSP94 binding protein
    • PSP94-binding protein
    • PSPBP
    see all
  • Function
    Putative serine protease inhibitor.
  • Tissue specificity
    Expressed in prostate, testis, ovary and intestine. Concentrates in prostate cancer patient's sera.
  • Sequence similarities
    Belongs to the CRISP family.
  • Post-translational
    modifications
    N-glycosylated.
  • Cellular localization
    Membrane.
  • Information by UniProt

Images

  • 12% SDS-PAGE of Human Peptidase inhibitor 16
    1. M.W. marker - 14, 21, 31, 45, 66, 97 kDa
    2. Reduced and boiled sample, 2µg / lane
    3. Non-reduced and non-boiled sample, 2µg / lane

References

ab117229 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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