Overview

Description

  • NatureRecombinant
  • SourceEscherichia coli
  • Amino Acid Sequence
    • AccessionQ9NZJ5
    • SpeciesHuman
    • SequenceNKYDSVSGEANDSSWNDIKNSGYISRYLTDFEPIQCLGRGGFGVVFEAKN KVDDCNYAIKRIRLPNRELAREKVMREVKALAKLEHPGIVRYFNAWLEAP PEKWQEKMDEIWLKDESTDWPLSSPSPMDAPSVKIRRMDPFATKEHIEII APSPQRSRSFSVGISCDQTSSSESQFSPLEFSGMDHEDISESVDAAYNLQ DSCLTDCDVEDGTMDGNDEGHSFELCPSEASPYVRSRERTSSSIVFEDSG CDNASSKEEPKTNRLHIGNHCANKLTAFKPTSSKSSSEATLSISPPRPTT LSLDLTKNTTEKLQPSSPKVYLYIQMQLCRKENLKDWMNGRCTIEERERS VCLHIFLQIAEAVEFLHSKGLMHRDLKPSNIFFTMDDVVKVGDFGLVTAM DQDEEEQTVLTPMPAYARHTGQVGTKLYMSPEQIHGNSYSHKVDIFSLGL ILFELLYPFSTQMERVRTLTDVRNLKFPPLFTQKYPCEYVMVQDMLSPSP MERPEAINIIENAVFEDLDFPGKTVLRQRSRSLSSSGTKHSRQSNNSHSP LPSN
    • Molecular weight115 kDa including tags
    • Amino acids563 to 1116

Specifications

Our Abpromise guarantee covers the use of ab101115 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Biological activityThe specific activity of ab101115 was determined to be 18 nmol/min/mg by activity assay.
  • Applications

    Western blot

    SDS-PAGE

    Functional Studies

  • Purity> 90 % SDS-PAGE.
    The purity was determined to be >90% by densitometry
  • FormLiquid
  • Additional notes

    ab61428 (Human Smad3 full length protein) can be utilized as a substrate for assessing Kinase activity

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    Preservative: None
    Constituents: 25% Glycerol, 50mM Tris HCl, 150mM Sodium chloride, 10mM Glutathione, 0.25mM DTT, 0.1mM EDTA, 0.1mM PMSF, pH 7.5

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

General Info

  • Alternative names
    • DKFZp781H1925
    • E2AK3_HUMAN
    • EC 2.7.11.1
    • Eif2ak3
    • Eukaryotic translation initiation factor 2 alpha kinase 3
    • Eukaryotic translation initiation factor 2-alpha kinase 3
    • Heme regulated EIF2 alpha kinase
    • HRI
    • HsPEK
    • Pancreatic eIF2 alpha kinase
    • Pancreatic eIF2-alpha kinase
    • PEK
    • PRKR like endoplasmic reticulum kinase
    • PRKR-like endoplasmic reticulum kinase
    • WRS
    see all
  • FunctionPhosphorylates the alpha subunit of eukaryotic translation-initiation factor 2 (EIF2), leading to its inactivation and thus to a rapid reduction of translational initiation and repression of global protein synthesis. Serves as a critical effector of unfolded protein response (UPR)-induced G1 growth arrest due to the loss of cyclin D1.
  • Tissue specificityUbiquitous. A high level expression is seen in secretory tissues.
  • Involvement in diseaseDefects in EIF2AK3 are the cause of Wolcott-Rallison syndrome (WRS) [MIM:226980]; also known as multiple epiphyseal dysplasia with early-onset diabetes mellitus. WRS is a rare autosomal recessive disorder, characterized by permanent neonatal or early infancy insulin-dependent diabetes and, at a later age, epiphyseal dysplasia, osteoporosis, growth retardation and other multisystem manifestations, such as hepatic and renal dysfunctions, mental retardation and cardiovascular abnormalities.
  • Sequence similaritiesBelongs to the protein kinase superfamily. Ser/Thr protein kinase family. GCN2 subfamily.
    Contains 1 protein kinase domain.
  • DomainThe lumenal domain senses perturbations in protein folding in the ER, probably through reversible interaction with HSPA5/BIP.
  • Post-translational
    modifications
    Autophosphorylated.
    N-glycosylated.
  • Cellular localizationEndoplasmic reticulum membrane.
  • Information by UniProt

Recombinant human PERK protein images

  • The putity of ab101115 was determined to be 90% by densitometry.

    Approximate MWt: 115kDa
  • The specific activity of ab101115 was determined to be 18 nmol/min/mg by activity assay.

References for Recombinant human PERK protein (ab101115)

ab101115 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab101115.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"