The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Biological activitySpecific Activity: 149 pmol/min/µg.
3.45 µg of Peroxiredoxin 2 was incubated in 50 mM Hepes (pH 7.0) containing 200 µM NADPH, 3 µM thioredoxin, and 1.5 µM thioredoxin reductase. The reaction mixture was incubated at 30C for 5 min, followed by the addition of 0.22 mM H2O2. NADPH oxidation was monitored (fluorescence decrease) for the next 10 min.
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Preparation and Storage
Stability and Storage
Shipped on Dry Ice. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Epididymis secretory sperm binding protein Li 2a
HEL S 2a
Natural killer cell enhancing factor B
Natural killer cell-enhancing factor B
Natural Killer Enhancing Factor B
Thiol Specific Antioxidant 1
Thiol specific antioxidant protein
Thiol-specific antioxidant protein
Thioredoxin Dependent Peroxide Reductase 1
Thioredoxin peroxidase 1
Thioredoxin-dependent peroxide reductase 1
FunctionInvolved in redox regulation of the cell. Reduces peroxides with reducing equivalents provided through the thioredoxin system. It is not able to receive electrons from glutaredoxin. May play an important role in eliminating peroxides generated during metabolism. Might participate in the signaling cascades of growth factors and tumor necrosis factor-alpha by regulating the intracellular concentrations of H(2)O(2).
Sequence similaritiesBelongs to the ahpC/TSA family. Contains 1 thioredoxin domain.