Recombinant human Peroxiredoxin 2 protein (ab79947)

Overview

Description

  • NatureRecombinant
  • SourceBaculovirus infected Sf9 cells
  • Amino Acid Sequence
    • SpeciesHuman

Specifications

Our Abpromise guarantee covers the use of ab79947 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Biological activitySpecific Activity: 149 pmol/min/µg. 3.45 µg of Peroxiredoxin 2 was incubated in 50 mM Hepes (pH 7.0) containing 200 µM NADPH, 3 µM thioredoxin, and 1.5 µM thioredoxin reductase. The reaction mixture was incubated at 30C for 5 min, followed by the addition of 0.22 mM H2O2. NADPH oxidation was monitored (fluorescence decrease) for the next 10 min.
  • Applications

    Functional Studies

    SDS-PAGE

    Western blot

  • Purity> 95 % SDS-PAGE.

  • FormLiquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on Dry Ice. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.

    Preservative: None
    Constituents: 50% Glycerol, 0.05% Tween 20, 3mM DTT, 25mM Tris HCl, 100mM Sodium chloride, pH 8

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

General Info

  • Alternative names
    • Epididymis secretory sperm binding protein Li 2a
    • HEL S 2a
    • MGC4104
    • Natural killer cell enhancing factor B
    • Natural killer cell-enhancing factor B
    • Natural Killer Enhancing Factor B
    • NKEF B
    • NKEF-B
    • NKEFB
    • Peroxiredoxin-2
    • PRDX 2
    • PRDX2
    • PRDX2_HUMAN
    • PrP
    • PRX2
    • PRXII
    • PTX1
    • TDPX1
    • Thiol Specific Antioxidant 1
    • Thiol specific antioxidant protein
    • Thiol-specific antioxidant protein
    • Thioredoxin Dependent Peroxide Reductase 1
    • Thioredoxin peroxidase 1
    • Thioredoxin-dependent peroxide reductase 1
    • Torin
    • TPX1
    • TSA
    see all
  • FunctionInvolved in redox regulation of the cell. Reduces peroxides with reducing equivalents provided through the thioredoxin system. It is not able to receive electrons from glutaredoxin. May play an important role in eliminating peroxides generated during metabolism. Might participate in the signaling cascades of growth factors and tumor necrosis factor-alpha by regulating the intracellular concentrations of H(2)O(2).
  • Sequence similaritiesBelongs to the ahpC/TSA family.
    Contains 1 thioredoxin domain.
  • Cellular localizationCytoplasm.
  • Information by UniProt

Recombinant human Peroxiredoxin 2 protein images

  • Lane 1: ab79947 on 14% SDS-PAGE, Coomassie staining, 10µg.
    Lane 2: Protein marker.
  • Anti-Peroxiredoxin 2 antibody (ab86295) at 1 µg/ml + Recombinant human Peroxiredoxin 2 protein (ab79947) at 0.01 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Exposure time : 2 minutes

References for Recombinant human Peroxiredoxin 2 protein (ab79947)

ab79947 has not yet been referenced specifically in any publications.

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