Overview

Description

  • Nature
    Recombinant
  • Source
    Escherichia coli
  • Amino Acid Sequence
    • Species
      Human
    • Sequence
      MGSSHHHHHH SSGLVPRGSH MSLSNKLTLD KLDVKGKRVV MRVDFNVPMK NNQITNNQRI KAAVPSIKFC LDNGAKSVVL MSHLGRPDGV PMPDKYSLEP VAVELKSLLG KDVLFLKDCV GPEVEKACAN PAAGSVILLE NLRFHVEEEG KGKDASGNKV KAEPAKIEAF RASLSKLGDV YVNDAFGTAH RAHSSMVGVN LPQKAGGFLM KKELNYFAKA LESPERPFLA ILGGAKVADK IQLINNMLDK VNEMIIGGGM AFTFLKVLNN MEIGTSLFDE EGAKIVKDLM SKAEKNGVKI TLPVDFVTAD KFDENAKTGQ ATVASGIPAG WMGLDCGPES SKKYAEAVTR AKQIVWNGPV GVFEWEAFAR GTKALMDEVV KATSRGCITI IGGGDTATCC AKWNTEDKVS HVSTGGGASL ELLEGKVLPG VDALSNI
    • Amino acids
      1 to 417

Specifications

Our Abpromise guarantee covers the use of ab87630 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    SDS-PAGE

    Western blot

  • Purity
    > 95 % SDS-PAGE.
    Purified by using conventional chromatography techniques.
  • Form
    Liquid
  • Additional notes
    Endotoxoin level: < 1.0 EU per 1 µg of protein (determined by LAL method)
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

    Preservative: None
    Constituents: 10% Glycerol, 20mM Tris, 1mM DTT, pH 8

General Info

  • Alternative names
    • Cell migration-inducing gene 10 protein
    • Epididymis secretory sperm binding protein Li 68p
    • HEL S 68p
    • MGC117307
    • MGC8947
    • MIG10
    • pgk1
    • PGK1_HUMAN
    • PGKA
    • Phosphoglycerate kinase 1
    • Primer recognition protein 2
    • PRP 2
    see all
  • Function
    In addition to its role as a glycolytic enzyme, it seems that PGK-1 acts as a polymerase alpha cofactor protein (primer recognition protein).
  • Pathway
    Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 2/5.
  • Involvement in disease
    Defects in PGK1 are the cause of phosphoglycerate kinase 1 deficiency (PGK1D) [MIM:300653]. It is a condition with a highly variable clinical phenotype that includes hemolytic anemia, rhabdomyolysis, myopathy and neurologic involvement. Patients can express one or more of these manifestations.
  • Sequence similarities
    Belongs to the phosphoglycerate kinase family.
  • Cellular localization
    Cytoplasm.
  • Information by UniProt

Recombinant Human PGK1 protein images

  • ab87630 on 15% SDS-PAGE (3µg)
  • Anti-PGK1 antibody (ab90787) at 1 µg/ml + Recombinant Human PGK1 protein (ab87630) at 0.1 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Exposure time : 30 seconds

References for Recombinant Human PGK1 protein (ab87630)

ab87630 has not yet been referenced specifically in any publications.

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