The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Specific activity: > 300 pmole/min/ug. Measured by the hydrolysis of Ubiquitin-AMC at pH 8.0, at 37°C. Activity Assay:
Prepare a 100ul of recombinant PGP9.5 protein with various concentrations (0.48ng, 0.9ng) in assay buffer and equilibrate to 37°C for 10 minutes. (Assay buffer: 50mM Tris-HCl, 0.5 mM EDTA, 1 mM DTT, 0.1 mg/ml Ovalbumin, pH 8.0.)
Add 50ul of 1uM Ubiquitin-AMC.
Read at excitation wavelengths 355nm and emission 460nm for 5 minutes.
- 96 Well Polystyrene Microplate, black
- Fluorescent plate reader (PerkinElmer, VICTOR X3)
Concentration information loading...
Preparation and Storage
Stability and Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Constituents: 20mM Tris HCl, 2mM EDTA, pH 8
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Epididymis luminal protein 117
Epididymis secretory protein Li 53
HEL S 53
Neuron cytoplasmic protein 9.5
Protein gene product 9.5
Ubiquitin C terminal esterase L1
Ubiquitin C terminal hydrolase
Ubiquitin C terminal hydrolase L1
Ubiquitin carboxyl terminal esterase L1
Ubiquitin carboxyl terminal hydrolase isozyme L1
Ubiquitin carboxyl-terminal hydrolase isozyme L1
Ubiquitin thioesterase L1
Ubiquitin thiolesterase L1
Ubiquitin-protein hydrolase involved both in the processing of ubiquitin precursors and of ubiquitinated proteins. This enzyme is a thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin. Also binds to free monoubiquitin and may prevent its degradation in lysosomes. The homodimer may have ATP-independent ubiquitin ligase activity.
Found in neuronal cell bodies and processes throughout the neocortex (at protein level). Expressed in neurons and cells of the diffuse neuroendocrine system and their tumors. Weakly expressed in ovary. Down-regulated in brains from Parkinson disease and Alzheimer disease patients.
Involvement in disease
Parkinson disease 5 Neurodegeneration with optic atrophy, childhood-onset
Belongs to the peptidase C12 family.
Cytoplasm. Endoplasmic reticulum membrane. About 30% of total UCHL1 is associated with membranes in brain.