Recombinant Human PHLPP2 protein (ab125556)

Overview

  • Product nameRecombinant Human PHLPP2 protein
  • Protein lengthProtein fragment

Description

  • NatureRecombinant
  • SourceEscherichia coli
  • Amino Acid Sequence
    • AccessionQ6ZVD8
    • SpeciesHuman
    • Molecular weight56 kDa including tags
    • Amino acids766 to 1043

Specifications

Our Abpromise guarantee covers the use of ab125556 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Western blot

    SDS-PAGE

  • Purity> 95 % Densitometry.
    ab125556 is >95% pure.
  • FormLiquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    pH: 7.50
    Constituents: 0.31% Glutathione, 0.002% PMSF, 0.004% DTT, 0.79% Tris HCl, 0.003% EDTA, 25% Glycerol, 0.88% Sodium chloride

General Info

  • Alternative names
    • KIAA0931
    • PH domain and leucine rich repeat protein phosphatase 2
    • PH domain leucine rich repeat protein phosphatase like
    • PH domain leucine-rich repeat-containing protein phosphatase 2
    • PH domain leucine-rich repeat-containing protein phosphatase-like
    • PHLP2_HUMAN
    • PHLPP like
    • PHLPP-like
    • Phlpp2
    • PHLPPL
    see all
  • FunctionProtein phosphatase that mediates dephosphorylation of 'Ser-473' of AKT1, 'Ser-660' of PRKCB isoform beta-II and 'Ser-657' of PRKCA. AKT1 regulates the balance between cell survival and apoptosis through a cascade that primarily alters the function of transcription factors that regulate pro- and antiapoptotic genes. Dephosphorylation of 'Ser-473' of AKT1 triggers apoptosis and decreases cell proliferation. Also controls the phosphorylation of AKT3. Dephosphorylation of PRKCA and PRKCB leads to their destabilization and degradation. Inhibits cancer cell proliferation and may act as a tumor suppressor.
  • Tissue specificityIn colorectal cancer tissue, expression is highest in the surface epithelium of normal colonic mucosa adjacent to the cancer tissue but is largely excluded from the crypt bases. Expression is lost or significantly decreased in 80% of tested tumors (at protein level).
  • Sequence similaritiesContains 22 LRR (leucine-rich) repeats.
    Contains 1 PH domain.
    Contains 1 PP2C-like domain.
  • Cellular localizationCytoplasm. Membrane. Nucleus. In colorectal cancer tissue, expression is concentrated in the cytoplasm and nucleus.
  • Information by UniProt

Recombinant Human PHLPP2 protein images

  • SDS PAGE analysis of ab125556 (PHLPP2 protein).

References for Recombinant Human PHLPP2 protein (ab125556)

ab125556 has not yet been referenced specifically in any publications.

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