Recombinant Human Phospho1 protein (ab117662)

Overview

Description

  • NatureRecombinant
  • SourceEscherichia coli
  • Amino Acid Sequence
    • AccessionQ8TCT1
    • SpeciesHuman
    • SequenceMRGSHHHHHH GMASMSGCFP VSGLRCLSRD GRMAAQGAPR FLLTFDFDET IVDENSDDSI VRAAPGQRLP ESLRATYREG FYNEYMQRVF KYLGEQGVRP RDLSAIYEAI PLSPGMSDLL QFVAKQGACF EVILISDANT FGVESSLRAA GHHSLFRRIL SNPSGPDARG LLALRPFHTH SCARCPANMC KHKVLSDYLR ERAHDGVHFE RLFYVGDGAN DFCPMGLLAG GDVAFPRRGY PMHRLIQEAQ KAEPSSFRAS VVPWETAADV RLHLQQVLKSC
    • Molecular weight31 kDa including tags
    • Amino acids1 to 267
    • TagsHis tag N-Terminus
    • Additional sequence informationHuman Phospho1 Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 295 amino acids and having a molecular mass of 31.3 kDa.

Specifications

Our Abpromise guarantee covers the use of ab117662 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    SDS-PAGE

  • Purity> 95 % SDS-PAGE.
    ab117662 is purified by proprietary chromatographic techniques.
  • FormLyophilised
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at -20°C.

  • ReconstitutionAdd 0.1M acetate buffer pH4 to a working concentration of 0.5mg/ml and let the lyophilized pellet dissolve completely. Add 0.1M acetate buffer pH4 to a working concentration of 0.5mg/ml and let the lyophilized pellet dissolve completely

General Info

  • Alternative names
    • PHOP1_HUMAN
    • Phosphatase orphan 1
    • Phospho 1
    • phospho1
    • Phosphoethanolamine/phosphocholine phosphatase
    see all
  • FunctionPhosphatase that has a high activity toward phosphoethanolamine (PEA) and phosphocholine (PCho). Involved in the generation of inorganic phosphate for bone mineralization.
  • Tissue specificityExpressed at sites of mineralization in bone and cartilage. Highly expressed in osteoblast cell line SaOS-2 which produces a mineralized matrix, but not in MG-63 cell line, which do not mineralize.
  • Sequence similaritiesBelongs to the HAD-like hydrolase superfamily. PHOSPHO family.
  • Information by UniProt

References for Recombinant Human Phospho1 protein (ab117662)

ab117662 has not yet been referenced specifically in any publications.

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