The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
% SDS-PAGE. ab117662 is purified by proprietary chromatographic techniques.
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Preparation and Storage
Stability and Storage
Shipped at 4°C. Store at -20°C.
ReconstitutionAdd 0.1M acetate buffer pH4 to a working concentration of 0.5mg/ml and let the lyophilized pellet dissolve completely. Add 0.1M acetate buffer pH4 to a working concentration of 0.5mg/ml and let the lyophilized pellet dissolve completely
Phosphatase orphan 1
FunctionPhosphatase that has a high activity toward phosphoethanolamine (PEA) and phosphocholine (PCho). Involved in the generation of inorganic phosphate for bone mineralization.
Tissue specificityExpressed at sites of mineralization in bone and cartilage. Highly expressed in osteoblast cell line SaOS-2 which produces a mineralized matrix, but not in MG-63 cell line, which do not mineralize.
Sequence similaritiesBelongs to the HAD-like hydrolase superfamily. PHOSPHO family.