Recombinant Human Phospho1 protein (ab63263)

Overview

Description

  • Nature
    Recombinant
  • Source
    Escherichia coli
  • Amino Acid Sequence
    • Species
      Human
    • Sequence
      MRGSHHHHHH GMAS-MSGCFP VSGLRCLSRD GRMAAQGAPR FLLTFDFDET IVDENSDDSI VRAAPGQRLP ESLRATYREG FYNEYMQRVF KYLGEQGVRP RDLSAIYEAI PLSPGMSDLL QFVAKQGACF EVILISDANT FGVESSLRAA GHHSLFRRIL SNPSGPDARG LLALRPFHTH SCARCPANMC KHKVLSDYLR ERAHDGVHFE RLFYVGDGAN DFCPMGLLAG GDVAFPRRGY PMHRLIQEAQ KAEPSSFRAS VVPWETAADV RLHLQQVLKS C

Specifications

Our Abpromise guarantee covers the use of ab63263 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Western blot

    SDS-PAGE

  • Purity
    > 95 % SDS-PAGE.
    Please filter the product by an appropriate sterile filter before using it in cell culture.
  • Form
    Lyophilised
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.

    Preservative: None
    Constituents: 0.03M Sodium acetate, pH 4

  • Reconstitution
    Add 0.1M Acetate buffer pH4 to prepare a working stock solution of approximately 0.5 mg/mL and let the lyophilized pellet dissolve completely. For conversion into higher pH value, we recommend intensive dilution by relevant buffer to a concentration of 10µg/mL. In higher concentrations the solubility of this antigen is limited.

General Info

  • Alternative names
    • PHOP1_HUMAN
    • Phosphatase orphan 1
    • Phospho 1
    • phospho1
    • Phosphoethanolamine/phosphocholine phosphatase
    see all
  • Function
    Phosphatase that has a high activity toward phosphoethanolamine (PEA) and phosphocholine (PCho). Involved in the generation of inorganic phosphate for bone mineralization.
  • Tissue specificity
    Expressed at sites of mineralization in bone and cartilage. Highly expressed in osteoblast cell line SaOS-2 which produces a mineralized matrix, but not in MG-63 cell line, which do not mineralize.
  • Sequence similarities
    Belongs to the HAD-like hydrolase superfamily. PHOSPHO family.
  • Information by UniProt

Images

  • LANE 1. M.W. marker – 14, 21, 31, 45, 66, 97 kDa. LANE 2. reduced and heated sample, 5µg/lane. LANE 3. non-reduced and non-heated sample, 5µg/lane

References

ab63263 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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