Recombinant Human Phospholamban protein (ab114227)

Overview

  • Product nameRecombinant Human Phospholamban protein
  • Protein lengthProtein fragment

Description

  • NatureRecombinant
  • SourceWheat germ
  • Amino Acid Sequence
    • AccessionP26678
    • SpeciesHuman
    • SequenceMEKVQYLTRSAIRRASTIEMPQQARQKLQN
    • Molecular weight29 kDa including tags
    • Amino acids1 to 30

Specifications

Our Abpromise guarantee covers the use of ab114227 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    ELISA

    SDS-PAGE

    Western blot

  • FormLiquid
  • Additional notesProtein concentration is above or equal to 0.05 mg/ml.
    Best use within three months from the date of receipt of this protein.
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    pH: 8.00
    Constituents: 0.3% Glutathione, 0.79% Tris HCl

General Info

  • Alternative names
    • Cardiac phospholamban
    • CMD1P
    • CMH18
    • PLB
    • Pln
    • PPLA_HUMAN
    see all
  • FunctionReversibly inhibits the activity of ATP2A2 in cardiac sarcoplasmic reticulum by decreasing the apparent affinity of the ATPase for Ca(2+). Modulates the contractility of the heart muscle in response to physiological stimuli via its effects on ATP2A2. Modulates calcium re-uptake during muscle relaxation and plays an important role in calcium homeostasis in the heart muscle. The degree of ATP2A2 inhibition depends on the oligomeric state of PLN. ATP2A2 inhibition is alleviated by PLN phosphorylation.
  • Tissue specificityHeart muscle (at protein level).
  • Involvement in diseaseCardiomyopathy, dilated 1P
    Cardiomyopathy, familial hypertrophic 18
  • Sequence similaritiesBelongs to the phospholamban family.
  • Post-translational
    modifications
    Phosphorylation by PKA abolishes the inhibition of ATP2A2-mediated calcium uptake. Phosphorylated at Thr-17 by CaMK2, and in response to beta-adrenergic stimulation. Phosphorylation by DMPK may stimulate sarcoplasmic reticulum calcium uptake in cardiomyocytes.
  • Cellular localizationEndoplasmic reticulum membrane. Sarcoplasmic reticulum membrane. Mitochondrion membrane. Membrane. Colocalizes with HAX1 at the endoplasmic reticulum (PubMed:17241641). Colocalizes with DMPK a the sarcoplasmic reticulum (PubMed:15598648).
  • Information by UniProt

Recombinant Human Phospholamban protein images

  • ab114227 analysed on a 12.5% SDS-PAGE gel stained with Coomassie Blue.

References for Recombinant Human Phospholamban protein (ab114227)

ab114227 has not yet been referenced specifically in any publications.

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