The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Preparation and Storage
Stability and Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
0.05M Acetate buffer pH 4.0
ReconstitutionAdd 0.1M Acetate buffer pH4 to prepare a working stock solution of approximately 0.5 mg/ml and let the lyophilized pellet dissolve completely. For conversion into higher pH value, we recommend intensive dilution by relevant buffer to a concentration of 10µg/ml. In higher concentrations the solubility of this antigen is limited. Product is not sterile! Please filter the product by an appropriate sterile filter before using it in the cell culture.
Group IIE secretory phospholipase A2
Group IIE secretory phospholipase A2 precursor
Phosphatidylcholine 2 acylhydrolase GIIE
Phosphatidylcholine 2-acylhydrolase 2E
Phospholipase A2 group IIE
FunctionPA2 catalyzes the calcium-dependent hydrolysis of the 2-acyl groups in 3-sn-phosphoglycerides. Has a preference for arachidonic-containing phospholipids.
Tissue specificityRestricted to the brain, heart, lung, and placenta.
Sequence similaritiesBelongs to the phospholipase A2 family.