Recombinant Human Phospholipase A2 IIE protein (ab63845)

Overview

  • Product name
    Recombinant Human Phospholipase A2 IIE protein
  • Protein length
    Full length protein

Description

  • Nature
    Recombinant
  • Source
    Escherichia coli
  • Amino Acid Sequence
    • Species
      Human
    • Sequence
      MRGSHHHHHH GMASHMNLVQ FGVMIEKMTG KSALQYNDYG CYCGIGGSHW PVDQTDWCCH AHDCCYGRLE KLGCEPKLEK YLFSVSERGI FCAGRTTCQR LTCECDKRAA LCFRRNLGTY NRKYAHYPNK LCTGPTPPC
    • Amino acids
      20 to 142

Specifications

Our Abpromise guarantee covers the use of ab63845 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Western blot

    SDS-PAGE

  • Purity
    > 95 % SDS-PAGE.

  • Form
    Lyophilised
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

    Preservative: None
    0.05M Acetate buffer pH 4.0

  • Reconstitution
    Add 0.1M Acetate buffer pH4 to prepare a working stock solution of approximately 0.5 mg/ml and let the lyophilized pellet dissolve completely. For conversion into higher pH value, we recommend intensive dilution by relevant buffer to a concentration of 10µg/ml. In higher concentrations the solubility of this antigen is limited. Product is not sterile! Please filter the product by an appropriate sterile filter before using it in the cell culture.

General Info

  • Alternative names
    • 2IIE
    • GIIE sPLA2
    • Group IIE secretory phospholipase A2
    • Group IIE secretory phospholipase A2 precursor
    • PA2GE_HUMAN
    • Phosphatidylcholine 2 acylhydrolase GIIE
    • Phosphatidylcholine 2-acylhydrolase 2E
    • Phospholipase A2 group IIE
    • Pla2g2e
    • sPLA
    • sPLA2-IIE
    see all
  • Function
    PA2 catalyzes the calcium-dependent hydrolysis of the 2-acyl groups in 3-sn-phosphoglycerides. Has a preference for arachidonic-containing phospholipids.
  • Tissue specificity
    Restricted to the brain, heart, lung, and placenta.
  • Sequence similarities
    Belongs to the phospholipase A2 family.
  • Cellular localization
    Secreted.
  • Information by UniProt

Images

  • 12% SDS-PAGE separation of ab63845. Molecular Weight: ~15.8 kDa.

    Lane 1. Molecular weight marker
    Lane 2. reduced and heated sample, 5µg/lane
    Lane 3. non-reduced and non-heated sample, 5µg/lane

References

ab63845 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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