Recombinant human Cytosolic Phospholipase A2 protein (ab198469)
Key features and details
- Expression system: Baculovirus infected insect cells
- Purity: >= 87% SDS-PAGE
- Active: Yes
- Tags: His-DDDDK tag C-Terminus
- Suitable for: Functional Studies, SDS-PAGE
Description
-
Product name
Recombinant human Cytosolic Phospholipase A2 protein -
Biological activity
Specific Activity: 57 pmol/min/µg.
Assay Conditions: PLA reaction was done using the 1-Hexadecanoyl-2-(1-prenedecanoyl)-sn-Glycero-3-phosphocholine
substrate. Enzyme reaction is conducted in 100 µl volume containing 50 mM Tris pH 8.0, 500 mM NaCl, 10 mM CaCl2, 0.5 mg/ml BSA, 10 µM substrate and 200 ng ab198469. Reaction progress was monitored at ex345/em395 for 10 minutes continuously at room temperature. -
Purity
>= 87 % SDS-PAGE.
Affinity purified. -
Expression system
Baculovirus infected insect cells -
Accession
-
Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
-
Species
Human -
Sequence
MSFIDPYQHIIVEHQYSHKFTVVVLRATKVTKGAFGDMLDTPDPYVELFI STTPDSRKRTRHFNNDINPVWNETFEFILDPNQENVLEITLMDANYVMDE TLGTATFTVSSMKVGEKKE VPFIFNQVTEMVLEMSLEVCSCPDLRFSM ALCDQEKTFRQQRKEHIRESMKKLLGPKN SEGLHSARDVPVVAILGSG GGFRAMVGFSGVMKALYESGILDCATYVAGLSGSTWYM STLYSHPDFP EKGPEEINEELMKNVSHNPLLLLTPQKVKRYVESLWKKKSSGQPVTFTD IFGMLIGETLIHNRMNTTLSSLKEKVNTAQCPLPLFTCLHVKPDVSELM FADWVEFSPYE IGMAKYGTFMAPDLFGSKFFMGTVVKKYEENPLHFLM GVWGSAFSILFNRVLGVSGSQ SRGSTMEEELENITTKHIVSNDSSDSD DESHEPKGTENEDAGSDYQSDNQASWIHRMI MALVSDSALFNTREGRA GKVHNFMLGLNLNTSYPLSPLSDFATQDSFDDDELDAAVAD PDEFERI YEPLDVKSKKIHVVDSGLTFNLPYPLILRPQRGVDLIISFDFSARPSDSS PPFK ELLLAEKWAKMNKLPFPKIDPYVFDREGLKECYVFKPKNPDMEK DCPTIIHFVLANINFR KYKAPGVPRETEEEKEIADFDIFDDPESPFST FNFQYPNQAFKRLHDLMHFNTLNNIDVI KEAMVESIEYRRQNPSRCSV SLSNVEARRFFNKEFLSKPKADYKDDDDKHHHHHH -
Predicted molecular weight
87 kDa including tags -
Amino acids
1 to 749 -
Tags
His-DDDDK tag C-Terminus -
Additional sequence information
GenBank Accession No. NM_024420
-
Associated products
-
Related Products
- Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody (ab1162)
- Anti-Cytosolic Phospholipase A2 antibody (ab139388)
- Anti-6X His tag® antibody [HIS.H8] (ab18184)
- Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [F-tag-01] (ab18230)
- Anti-6X His tag® antibody [4D11] (ab5000)
- Anti-Cytosolic Phospholipase A2 antibody (ab58375)
- Anti-6X His tag® antibody (ab9108)
Specifications
Our Abpromise guarantee covers the use of ab198469 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
-
Applications
Functional Studies
SDS-PAGE
-
Form
Liquid -
Concentration information loading...
Preparation and Storage
-
Stability and Storage
Shipped on Dry Ice. Store at -80°C. Avoid freeze / thaw cycle.
pH: 8.00
Constituents: 0.63% Tris HCl, 0.64% Sodium chloride, 0.02% Potassium chloride, 20% Glycerol (glycerin, glycerine)
80 µg/ml DDDDK peptideThis product is an active protein and may elicit a biological response in vivo, handle with caution.
General Info
-
Alternative names
- Calcium dependent phospholipid binding protein
- CPLA 2
- cPLA2
see all -
Function
Selectively hydrolyzes arachidonyl phospholipids in the sn-2 position releasing arachidonic acid. Together with its lysophospholipid activity, it is implicated in the initiation of the inflammatory response. -
Tissue specificity
Expressed in various tissues such as macrophages, platelets, neutrophils, fibroblasts and lung endothelium. -
Sequence similarities
Contains 1 C2 domain.
Contains 1 PLA2c domain. -
Domain
The N-terminal C2 domain associates with lipid membranes upon calcium binding. It modulates enzyme activity by presenting the active site to its substrate in response to elevations of cytosolic Ca(2+). -
Post-translational
modificationsActivated by phosphorylation at both Ser-505 and Ser-727. -
Cellular localization
Cytoplasm. Cytoplasmic vesicle. Translocates to membrane vesicles in a calcium-dependent fashion. - Information by UniProt
Images
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
-
SDS download
-
Datasheet download
References (0)
ab198469 has not yet been referenced specifically in any publications.