Recombinant Human Phospholipase A2 XII protein (ab63848)

Overview

Description

  • NatureRecombinant
  • SourceEscherichia coli
  • Amino Acid Sequence
    • SpeciesHuman
    • SequenceMRGSHHHHHH GMASHMQEQA QTTDWRATLK TIRNGVHKID TYLNAALDLL GGEDGLCQYK CSDGSKPFPR YGYKPSPPNG CGSPLFGVHL NIGIPSLTKC CNQHDRCYET CGKSKNDCDE EFQYCLSKIC RDVQKTLGLT QHVQACETTV ELLFDSVIHL GCKPYLDSQR AACRCHYEEK TDL
    • Amino acids23 to 189

Specifications

Our Abpromise guarantee covers the use of ab63848 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Western blot

  • Purity> 95 % SDS-PAGE.

  • FormLyophilised
  • Additional notesProduct is NOT sterile! Please filter the product by an appropriate sterile filter before using it in cell culture.
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.

    Preservative: None
    Constituents: 0.01M Tris buffer, pH 8.6

  • ReconstitutionAdd deionized water to prepare a working stock solution of approximately 0.5 mg/ml and let the lyophilized pellet dissolve completely.

General Info

  • Alternative names
    • EC 3.1.1.4
    • Group XII secreted phospholipase A2
    • Group XIIA secreted phospholipase A2
    • Group XIIA secretory phospholipase A2
    • Group XIIA secretory phospholipase A2 precursor
    • GXII
    • GXII sPLA2
    • PG12A_HUMAN
    • phosphatidylcholine 2 acylhydrolase 12A
    • Phosphatidylcholine 2 acylhydrolase GXII
    • Phosphatidylcholine 2-acylhydrolase 12A
    • Phospholipase A2 group XIIA
    • PLA2G12
    • Pla2g12a
    • ROSSY
    • sPLA2 XII
    • sPLA2-XII
    see all
  • FunctionPA2 catalyzes the calcium-dependent hydrolysis of the 2-acyl groups in 3-sn-phosphoglycerides. Does not exhibit detectable activity toward sn-2-arachidonoyl- or linoleoyl-phosphatidylcholine or -phosphatidylethanolamine.
  • Tissue specificityAbundantly expressed in heart, skeletal muscle, kidney, liver and pancreas.
  • Sequence similaritiesBelongs to the phospholipase A2 family.
  • Cellular localizationSecreted. Cytoplasm.
  • Information by UniProt

Recombinant Human Phospholipase A2 XII protein images

  • 12% SDS-PAGE separation of ab63848. Molecular Weight: ~20.6 kDa.

    Lane 1. Molecular weight marker
    Lane 2. reduced and heated sample, 5µg/lane
    Lane 3. non-reduced and non-heated sample, 5µg/lane

References for Recombinant Human Phospholipase A2 XII protein (ab63848)

ab63848 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab63848.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"