Overview

  • Product nameRecombinant Human PHPT1 protein
  • Protein lengthFull length protein

Description

  • NatureRecombinant
  • SourceEscherichia coli
  • Amino Acid Sequence
    • AccessionQ9NRX4
    • SpeciesHuman
    • SequenceMGSSHHHHHHSSGLVPRGSHMAVADLALIPDVDIDSDGVFKYVLIRVHSA PRSGAPAAESKEIVRGYKWAEYHADIYDKVSGDMQKQGCDCECLGGGRIS HQSQDKKIHVYGYSMAYGPAQHAISTEKIKAKYPDYEVTWANDGY
    • Molecular weight16 kDa
    • Amino acids1 to 125
    • TagsHis tag N-Terminus

Specifications

Our Abpromise guarantee covers the use of ab99865 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    SDS-PAGE

    Mass Spectrometry

  • Mass spectrometry
    MALDI-TOF
  • Purity> 95 % SDS-PAGE.
    ab99865 is purified using conventional chromatography techniques.
  • FormLiquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

    Preservative: None
    Constituents: 10% Glycerol, 0.2M Sodium chloride, 20mM Tris HCl, 2mM DTT, pH 8.0

General Info

  • Alternative names
    • PHP14
    • 14 kDa phosphohistidine phosphatase
    • CGI 202
    • HSPC141
    • Phosphohistidine phosphatase 1
    • PHPT 1
    • Protein janus A homolog
    see all
  • RelevancePHPT1 is an EDTA-insensitive phosphohistidine phosphatase that catalyzes the dephosphorylation of phosphopeptide I. Protein histidine phosphorylation and dephosphorylation in eukaryotes has been sparsely studied compared to protein serine/threonine and tyrosine phosphorylation, although protein histidine phosphorylation is estimated to account for about 6% of total protein phosphorylation in eukaryotes. Ek et al. (2002) detected no activity of PHPT1 towards phosphoserine, phosphothreonine, or phosphotyrosine peptides.
  • Cellular localizationCytoplasmic

Recombinant Human PHPT1 protein images

  • 15% SDS-PAGE showing ab99865 (3µg).

References for Recombinant Human PHPT1 protein (ab99865)

ab99865 has not yet been referenced specifically in any publications.

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