Recombinant human PI 3 Kinase p110 alpha + PI 3 kinase p85 alpha protein (ab90730)

Overview

Description

  • NatureRecombinant
  • SourceBaculovirus infected Sf9 cells
  • Amino Acid Sequence
    • SpeciesHuman

Specifications

Our Abpromise guarantee covers the use of ab90730 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Biological activityActivity: 9,900 units/mg 1 unit equals 1 picomole phosphate transferred to PIP2 per min. Activity test: ATP depletion assay, PI as substrate, 1 µM ATP.
  • Applications

    Functional Studies

    SDS-PAGE

  • Purity> 90 % SDS-PAGE.

  • FormLiquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    Preservative: None
    Constituents: 10% Glycerol, 0.05% Brij 35, 50mM Tris HCl, 300mM Sodium chloride, pH 7.9

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

General Info

  • Alternative names
    • GRB1
    • MGC142161
    • MGC142163
    • p110 alpha
    • p85
    • p85 alpha
    • Phosphatidylinositol 3 kinase 85 kDa regulatory subunit alpha
    • Phosphatidylinositol 3 kinase associated p 85 alpha
    • Phosphatidylinositol 3 kinase catalytic 110 KD alpha
    • Phosphatidylinositol 3 kinase catalytic alpha polypeptide
    • Phosphatidylinositol 3 kinase regulatory 1
    • Phosphatidylinositol 3 kinase regulatory subunit alpha
    • Phosphatidylinositol 3 kinase regulatory subunit polypeptide 1
    • Phosphatidylinositol 4 5 bisphosphate 3 kinase 110 kDa catalytic subunit alpha
    • Phosphatidylinositol 4 5 bisphosphate 3 kinase catalytic subunit alpha isoform
    • Phosphoinositide 3 kinase catalytic alpha polypeptide
    • Phosphoinositide 3 kinase regulatory subunit 1
    • Phosphoinositide 3 kinase regulatory subunit 1 (alpha)
    • PI3 kinase p110 subunit alpha
    • PI3 kinase regulatory subunit alpha
    • PI3 kinase subunit alpha
    • PI3 kinase subunit p85 alpha
    • PI3K
    • PI3K alpha
    • PI3K regulatory subunit alpha
    • PIK3CA
    • PIK3R1
    • PtdIns 3 kinase p110
    • PtdIns 3 kinase regulatory subunit alpha
    • PtdIns 3 kinase regulatory subunit p85 alpha
    • PtdIns 3 kinase subunit alpha
    • PtdIns 3 kinase subunit p110 alpha
    see all
  • RelevancePhosphoinositide 3-kinases (PI3Ks) phosphorylate phosphatidylinositols (PIs) at their 3´ OH position generating lipid second messengers and thereby regulate numerous biological processes including cell growth, differentiation, survival, proliferation, migration and metabolism. On the basis of structural similarities and substrate specificity, the PI3K family can be subdivided into three classes termed I, II, and III. All human class I members are heterodimers consisting of a catalytic subunit (MW approx. 110 kDa) and a non-catalytic subunit (MW 50, 55, 85, or 101 kDa) and are known to phosphorylate phosphatidylinositol (PI), phosphatidylinositol-4-mono-phosphate (PIP) and phosphatidylinositol-4,5-bisphosphate (PIP2) in vitro. The class I members can be further subdivided into class IA and IB PI3Ks. Class IA exists in three isoforms (p110alpha, p110beta and p110delta whereas the only class IB member is termed p110gamma. Class IA PI3Ks are activated by adaptor proteins such as Ras or BCAP, or tyrosine-kinase-associated receptors including antigen, co-stimulatory and cytokine receptors (e.g. CD19, CD28, Insulin receptor, EGFR, and PDGFR). p110gamma is activated by G-protein-coupled receptors (GPCRs). Effectors of class I PI3Ks are pleckstrin homology domain proteins such as Akt/PKB, BTK, TEC, ITK, BAM32, and small GTPases (e.g. Cdc42, Rac, or Ras). The action of PI3Ks is regulated by the phosphatidylinositol- 3,4,5-trisphosphate phosphatases SHIP and PTEN.

Recombinant human PI 3 Kinase p110 alpha + PI 3 kinase p85 alpha protein images

  • SDS-PAGE analysis of 3µg of ab90730
  • ATP depletion assay, PI as substrate, 1 µM ATP

References for Recombinant human PI 3 Kinase p110 alpha + PI 3 kinase p85 alpha protein (ab90730)

ab90730 has not yet been referenced specifically in any publications.

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