Recombinant Human PI 3 Kinase p85 alpha protein (ab84769)
Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: > 95% Densitometry
- Tags: His tag N-Terminus
- Suitable for: WB, SDS-PAGE
Description
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Product name
Recombinant Human PI 3 Kinase p85 alpha protein
See all PI 3 Kinase p85 alpha proteins and peptides -
Purity
> 95 % Densitometry.
Affinity purified. -
Expression system
Baculovirus infected Sf9 cells -
Accession
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Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Sequence
MSAEGYQYRALYDYKKEREEDIDLHLGDILTVNKGSLVALGFSDGQEARP EEIGWLNGYN ETTGERGDFPGTYVEYIGRKKISPPTPKPRPPRPLPVA PGSSKTEADVEQQALTLPDLAE QFAPPDIAPPLLIKLVEAIEKKGLEC STLYRTQSSSNLAELRQLLDCDTPSVDLEMIDVH VLADAFKRYLLDLP NPVIPAAVYSEMISLAPEVQSSEEYIQLLKKLIRSPSIPHQYWLTL QY LLKHFFKLSQTSSKNLLNARVLSEIFSPMLFRFSAASSDNTENLIKVIEI LISTEWNE RQPAPALPPKPPKPTTVANNGMNNNMSLQDAEWYWGDISR EEVNEKLRDTADGTFLVRDA STKMHGDYTLTLRKGGNNKLIKIFHRDG KYGFSDPLTFSSVVELINHYRNESLAQYNPKL DVKLLYPVSKYQQDQV VKEDNIEAVGKKLHEYNTQFQEKSREYDRLYEEYTRTSQEIQMK RTAI EAFNETIKIFEEQCQTQERYSKEYIEKFKREGNEKEIQRIMHNYDKLKSR ISEIID SRRRLEEDLKKQAAEYREIDKRMNSIKPDLIQLRKTRDQYLM WLTQKGVRQKKLNEWLGN ENTEDQYSLVEDDEDLPHHDEKTWNVGSSN RNKAENLLRGKRDGTFLVRESSKQGCYACS VVVDGEVKHCVINKTATG YGFAEPYNLYSSLKELVLHYQHTSLVQHNDSLNVTLAYPVYA QQRR -
Amino acids
1 to 724 -
Tags
His tag N-Terminus
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Associated products
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Related Products
Specifications
Our Abpromise guarantee covers the use of ab84769 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
Western blot
SDS-PAGE
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Form
Liquid -
Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
pH: 7.00
Preservative: 1.02% Imidazole
Constituents: 0.00174% PMSF, 0.82% Sodium phosphate, 0.0308% DTT, 25% Glycerol (glycerin, glycerine), 1.74% Sodium chloride
General Info
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Alternative names
- GRB1
- p85
- p85 alpha
see all -
Function
Binds to activated (phosphorylated) protein-Tyr kinases, through its SH2 domain, and acts as an adapter, mediating the association of the p110 catalytic unit to the plasma membrane. Necessary for the insulin-stimulated increase in glucose uptake and glycogen synthesis in insulin-sensitive tissues. -
Tissue specificity
Isoform 2 is expressed in skeletal muscle and brain, and at lower levels in kidney and cardiac muscle. Isoform 2 and isoform 4 are present in skeletal muscle (at protein level). -
Sequence similarities
Belongs to the PI3K p85 subunit family.
Contains 1 Rho-GAP domain.
Contains 2 SH2 domains.
Contains 1 SH3 domain. -
Domain
The SH3 domain mediates the binding to CBLB, and to HIV-1 Nef. -
Post-translational
modificationsPolyubiquitinated in T-cells by CBLB; which does not promote proteasomal degradation but impairs association with CD28 and CD3Z upon T-cell activation.
Phosphorylated. Dephosphorylated by PTPRJ. - Information by UniProt
Images
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All lanes : Anti-PI 3 Kinase p85 alpha antibody (ab71925) at 1 µg/ml
Lane 1 :Recombinant Human PI 3 Kinase p85 alpha protein (ab84769) at 0.1 µg
Lane 2 :Recombinant Human PI 3 Kinase p85 alpha protein (ab84769) at 0.01 µg
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Exposure time: 10 seconds -
SDS-PAGE showing ab84769 at approximately 86kDa.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab84769 has not yet been referenced specifically in any publications.