Recombinant Human PI 3 Kinase p85 beta protein (ab125568)

Overview

Description

  • Nature
    Recombinant
  • Source
    Baculovirus infected Sf9 cells
  • Amino Acid Sequence
    • Accession
    • Species
      Human
    • Molecular weight
      88 kDa including tags
    • Amino acids
      1 to 728
    • Tags
      His tag N-Terminus

Specifications

Our Abpromise guarantee covers the use of ab125568 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Western blot

    SDS-PAGE

  • Purity
    > 85 % Densitometry.
    Purity was determined to be >85% by densitometry.
  • Form
    Liquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    pH: 7.00
    Preservative: 1.02% Imidazole
    Constituents: 0.002% PMSF, 0.81% Sodium phosphate, 0.004% DTT, 25% Glycerol, 1.75% Sodium chloride

General Info

  • Alternative names
    • p85
    • p85 beta
    • P85B
    • P85B_HUMAN
    • Phosphatidylinositol 3 kinase
    • Phosphatidylinositol 3 kinase regulatory beta subunit
    • Phosphatidylinositol 3 kinase regulatory subunit beta
    • Phosphatidylinositol 3 kinase regulatory subunit polypeptide 2
    • Phosphatidylinositol 3 kinase, regulatory subunit, polypeptide 2 (p85 beta)
    • Phosphatidylinositol 3-kinase 85 kDa regulatory subunit beta
    • Phosphatidylinositol 3-kinase regulatory subunit beta
    • Phosphoinositide 3 kinase regulatory subunit 2
    • Phosphoinositide 3 kinase regulatory subunit 2 (beta)
    • Phosphoinositide 3 kinase regulatory subunit polypeptide 2
    • Phosphoinositide 3 kinase regulatory subunit polypeptide 2 (p85 beta)
    • Phosphoinositide 3 kinase, regulatory subunit 2 (beta)
    • Phosphoinositide 3 kinase, regulatory subunit 2 (p85 beta)
    • PI3 kinase p85 beta subunit
    • PI3 kinase p85 subunit beta
    • PI3-kinase regulatory subunit beta
    • PI3-kinase subunit p85-beta
    • PI3K
    • PI3K regulatory subunit beta
    • PIK3R 2
    • PIK3R2
    • PtdIns 3 kinase p85 beta
    • PtdIns-3-kinase regulatory subunit beta
    • PtdIns-3-kinase regulatory subunit p85-beta
    see all
  • Function
    Regulatory subunit of phosphoinositide-3-kinase (PI3K), a kinase that phosphorylates PtdIns(4,5)P2 (Phosphatidylinositol 4,5-bisphosphate) to generate phosphatidylinositol 3,4,5-trisphosphate (PIP3). PIP3 plays a key role by recruiting PH domain-containing proteins to the membrane, including AKT1 and PDPK1, activating signaling cascades involved in cell growth, survival, proliferation, motility and morphology. Binds to activated (phosphorylated) protein-tyrosine kinases, through its SH2 domain, and acts as an adapter, mediating the association of the p110 catalytic unit to the plasma membrane. Indirectly regulates autophagy (PubMed:23604317). Promotes nuclear translocation of XBP1 isoform 2 in a ER stress- and/or insulin-dependent manner during metabolic overloading in the liver and hence plays a role in glucose tolerance improvement.
  • Involvement in disease
    Megalencephaly-polymicrogyria-polydactyly-hydrocephalus syndrome 1
  • Sequence similarities
    Belongs to the PI3K p85 subunit family.
    Contains 1 Rho-GAP domain.
    Contains 2 SH2 domains.
    Contains 1 SH3 domain.
  • Domain
    The SH2 2 domain is required for interaction with FBXL2 and PTPN13.
  • Post-translational
    modifications
    Phosphorylated in response to signaling from activated receptor-type protein kinases (PubMed:19690332, PubMed:20068231). Dephosphorylated by PTPRJ (PubMed:18348712). Dephosphorylated at Tyr-655 by PTPN13. Phosphorylation of Tyr-655 impairs while its dephosphorylation promotes interaction with FBXL2 and SCF(FBXL2)-mediated polyubiquitination (PubMed:23604317).
    Ubiquitinated. Polyubiquitination by the SCF(FBXL2) complex probably promotes proteasomal degradation of PIK3R2.
  • Information by UniProt

Images

  • SDS-PAGE analysis of ab125568.

References

ab125568 has not yet been referenced specifically in any publications.

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