Recombinant human PI 4 Kinase type 2 beta protein (ab105202)

Overview

  • Product name
    Recombinant human PI 4 Kinase type 2 beta protein
  • Protein length
    Full length protein

Description

  • Nature
    Recombinant
  • Source
    Baculovirus infected Sf9 cells
  • Amino Acid Sequence
    • Accession
    • Species
      Human
    • Molecular weight
      84 kDa including tags
    • Amino acids
      1 to 481

Specifications

Our Abpromise guarantee covers the use of ab105202 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Biological activity
    The Specific activity of ab105202 was determined to be 218 nmol/min/mg.
  • Applications

    SDS-PAGE

    Western blot

    Functional Studies

  • Purity
    > 90 % SDS-PAGE.
    Purity was determined to be >90% by densitometry.
  • Form
    Liquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    Preservative: None
    Constituents: 25% Glycerol, 50mM Tris HCl, 150mM Sodium chloride, 10mM Glutathione, 0.25mM DTT, 0.1mM EDTA, 0.1mM PMSF, pH 7.5

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

General Info

  • Alternative names
    • 2610042N09Rik
    • 4933409G22Rik
    • FLJ11105
    • P4K2B_HUMAN
    • Phosphatidylinositol 4 kinase type 2 beta
    • Phosphatidylinositol 4 kinase type II beta
    • Phosphatidylinositol 4-kinase type 2-beta
    • Phosphatidylinositol 4-kinase type II-beta
    • PI4K 2 beta
    • PI4K 2b
    • PI4K II beta
    • PI4K IIb
    • Pi4k2b
    • PI4KII-BETA
    • PI4KIIb
    • PIK42B
    • Type II phosphatidylinositol 4 kinase beta isoform
    see all
  • Function
    Together with PI4K2A and the type III PI4Ks (PIK4CA and PIK4CB) it contributes to the overall PI4-kinase activity of the cell. This contribution may be especially significant in plasma membrane, endosomal and Golgi compartments. The phosphorylation of phosphatidylinositol (PI) to PI4P is the first committed step in the generation of phosphatidylinositol 4,5-bisphosphate (PIP2), a precursor of the second messenger inositol 1,4,5-trisphosphate (InsP3). Contributes to the production of InsP3 in stimulated cells and is likely to be involved in the regulation of vesicular trafficking.
  • Tissue specificity
    Widely expressed.
  • Sequence similarities
    Belongs to the PI3/PI4-kinase family. Type II PI4K subfamily.
    Contains 1 PI3K/PI4K domain.
  • Cellular localization
    Cytoplasm. Membrane. Mostly cytoplasmic but also found associated with the plasma membrane, the Golgi and endosomes. Compared to PI4K2A, a larger fraction of PI4K2B is cytosolic due to a smaller extent of palmitoylation. Translocates to membranes where it is recruited by PDGF stimulation by a Rac-GTP-dependent mechanism.
  • Information by UniProt

Images

  • The Specific activity of ab105202 was determined to be 218 nmol/min/mg.
  • SDS-PAGE showing ab105202 at approximately 84kDa.

References

ab105202 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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