Recombinant human PI 4 Kinase type 2 beta protein (ab105202)

Overview

  • Product nameRecombinant human PI 4 Kinase type 2 beta protein
  • Protein lengthFull length protein

Description

  • NatureRecombinant
  • SourceBaculovirus infected Sf9 cells
  • Amino Acid Sequence
    • AccessionQ8TCG2
    • SpeciesHuman
    • Molecular weight84 kDa including tags
    • Amino acids1 to 481

Specifications

Our Abpromise guarantee covers the use of ab105202 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Biological activityThe Specific activity of ab105202 was determined to be 218 nmol/min/mg.
  • Applications

    SDS-PAGE

    Western blot

    Functional Studies

  • Purity> 90 % SDS-PAGE.
    Purity was determined to be >90% by densitometry.
  • FormLiquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    Preservative: None
    Constituents: 25% Glycerol, 50mM Tris HCl, 150mM Sodium chloride, 10mM Glutathione, 0.25mM DTT, 0.1mM EDTA, 0.1mM PMSF, pH 7.5

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

General Info

  • Alternative names
    • 2610042N09Rik
    • 4933409G22Rik
    • FLJ11105
    • P4K2B_HUMAN
    • Phosphatidylinositol 4 kinase type 2 beta
    • Phosphatidylinositol 4 kinase type II beta
    • Phosphatidylinositol 4-kinase type 2-beta
    • Phosphatidylinositol 4-kinase type II-beta
    • PI4K 2 beta
    • PI4K 2b
    • PI4K II beta
    • PI4K IIb
    • Pi4k2b
    • PI4KII-BETA
    • PI4KIIb
    • PIK42B
    • Type II phosphatidylinositol 4 kinase beta isoform
    see all
  • FunctionTogether with PI4K2A and the type III PI4Ks (PIK4CA and PIK4CB) it contributes to the overall PI4-kinase activity of the cell. This contribution may be especially significant in plasma membrane, endosomal and Golgi compartments. The phosphorylation of phosphatidylinositol (PI) to PI4P is the first committed step in the generation of phosphatidylinositol 4,5-bisphosphate (PIP2), a precursor of the second messenger inositol 1,4,5-trisphosphate (InsP3). Contributes to the production of InsP3 in stimulated cells and is likely to be involved in the regulation of vesicular trafficking.
  • Tissue specificityWidely expressed.
  • Sequence similaritiesBelongs to the PI3/PI4-kinase family. Type II PI4K subfamily.
    Contains 1 PI3K/PI4K domain.
  • Cellular localizationCytoplasm. Membrane. Mostly cytoplasmic but also found associated with the plasma membrane, the Golgi and endosomes. Compared to PI4K2A, a larger fraction of PI4K2B is cytosolic due to a smaller extent of palmitoylation. Translocates to membranes where it is recruited by PDGF stimulation by a Rac-GTP-dependent mechanism.
  • Information by UniProt

Recombinant human PI 4 Kinase type 2 beta protein images

  • The Specific activity of ab105202 was determined to be 218 nmol/min/mg.
  • SDS-PAGE showing ab105202 at approximately 84kDa.

References for Recombinant human PI 4 Kinase type 2 beta protein (ab105202)

ab105202 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab105202.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"