Recombinant human PI4 kinase beta protein (ab105199)

Overview

Description

  • NatureRecombinant
  • SourceBaculovirus infected Sf9 cells
  • Amino Acid Sequence
    • AccessionQ9UBF8-2
    • SpeciesHuman
    • Molecular weight120 kDa including tags
    • Amino acids1 to 801

Associated products

Specifications

Our Abpromise guarantee covers the use of ab105199 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Biological activityThe Specific activity of ab105199 was determined to be 32 nmol/min/mg.
  • Applications

    SDS-PAGE

    Western blot

    Functional Studies

  • Purity> 90 % SDS-PAGE.
    Purity was determined to be >90% by densitometry.
  • FormLiquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    Preservative: None
    Constituents: 25% Glycerol, 50mM Tris HCl, 150mM Sodium chloride, 10mM Glutathione, 0.25mM DTT, 0.1mM EDTA, 0.1mM PMSF, pH 7.5

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

General Info

  • Alternative names
    • DKFZp686O1820
    • FLJ30129
    • NPIK
    • Phosphatidylinositol 4 kinase beta
    • Phosphatidylinositol 4 kinase catalytic beta
    • Phosphatidylinositol 4 kinase catalytic beta polypeptide
    • Phosphatidylinositol 4 kinase wortmannin sensitive
    • Phosphatidylinositol 4-kinase beta
    • PI4K BETA
    • PI4K-beta
    • PI4K92
    • PI4KB
    • PI4KB_HUMAN
    • PI4Kbeta
    • PI4KIIIbeta
    • PIK4CB
    • PtdIns 4 kinase
    • PtdIns 4-kinase beta
    • Type III phosphatidylinositol 4 kinase beta
    see all
  • FunctionPhosphorylates phosphatidylinositol (PI) in the first committed step in the production of the second messenger inositol-1,4,5,-trisphosphate (PIP). May regulate Golgi disintegration/reorganization during mitosis, possibly via its phosphorylation. Involved in Golgi-to-plasma membrane trafficking.
  • Tissue specificityWidely expressed with highest levels in heart, skeletal muscle, pancreas, testis and ovary. Weakly expressed in liver.
  • Sequence similaritiesBelongs to the PI3/PI4-kinase family. Type III PI4K subfamily.
    Contains 1 PI3K/PI4K domain.
    Contains 1 PIK helical domain.
  • Cellular localizationEndomembrane system. Mitochondrion outer membrane. Rough endoplasmic reticulum membrane. Golgi apparatus. Cytoplasm, perinuclear region. Found in the outer membrane of mitochondria and membranes of the rough endoplasmic reticulum. Recruited to the Golgi complex by the small GTPase ARF to stimulate the synthesis of phosphatidylinositol 4,5-bisphosphate (PIP2) on the Golgi complex.
  • Information by UniProt

Recombinant human PI4 kinase beta protein images

  • The Specific activity of ab105199 was determined to be 32 nmol/min/mg.
  • SDS-PAGE showing ab105199 at approximately 120kDa.

References for Recombinant human PI4 kinase beta protein (ab105199)

ab105199 has not yet been referenced specifically in any publications.

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