The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Preparation and Storage
Stability and Storage
Shipped at 4°C. Store at -20°C.
Constituents: 0.32% Tris HCl, 0.58% Sodium chloride
ReconstitutionReconstitute with water to desired concentration.
E3 SUMO protein ligase PIAS 3
E3 SUMO protein ligase PIAS3
E3 SUMO-protein ligase PIAS3
Protein inhibitor of activated STAT 3
Protein inhibitor of activated STAT protein 3
Protein inhibitor of activated STAT3
Zinc finger MIZ type containing 5
FunctionFunctions as an E3-type small ubiquitin-like modifier (SUMO) ligase, stabilizing the interaction between UBE2I and the substrate, and as a SUMO-tethering factor. Plays a crucial role as a transcriptional coregulation in various cellular pathways, including the STAT pathway and the steroid hormone signaling pathway. Involved in regulating STAT3 signaling via inhibiting STAT3 DNA-binding and suppressing cell growth. Enhances the sumoylation of MTA1 and may participate in its paralog-selective sumoylation (PubMed:21965678, PubMed:9388184). Sumoylates CCAR2 which promotes its interaction with SIRT1 (PubMed:25406032). Diminishes the sumoylation of ZFHX3 by preventing the colocalization of ZFHX3 with SUMO1 in the nucleus (PubMed:24651376).
Tissue specificityWidely expressed.
PathwayProtein modification; protein sumoylation.
Sequence similaritiesBelongs to the PIAS family. Contains 1 PINIT domain. Contains 1 SAP domain. Contains 1 SP-RING-type zinc finger.
DomainThe PINIT domain of PIAS3 is required for STAT3-PIAS3 interaction and for transloaction to the nucleus. The LXXLL motif is a transcriptional coregulator signature.
Cellular localizationCytoplasm. Nucleus. Nucleus speckle. Colocalizes with MITF in the nucleus. Colocalizes with GFI1 in nuclear dots. Colocalizes with SUMO1 in nuclear granules.