Recombinant human PIK3CB + PIK3R1 protein (ab80378)

Overview

Description

  • NatureRecombinant
  • SourceBaculovirus infected Sf9 cells
  • Amino Acid Sequence
    • SpeciesHuman

Specifications

Our Abpromise guarantee covers the use of ab80378 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Biological activitySpecific Activity: 7.9 pmol/min/µg. A 50 µl kinase reaction is conducted in a buffer containing 10 mM Tris-HCl (pH 7.5), 50 mM NaCl, 5 mM MgCl2, 5 µM ATP, 17 µg Phosphoinositol-4,5-Bisphosphate, and 150 ng enzyme for 90 minutes at room temperature.
  • Applications

    Functional Studies

    SDS-PAGE

  • FormLiquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on Dry Ice. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.

    Preservative: None
    Constituents: 30% Glycerol, 0.05% Tween 20, 3mM DTT, 25mM Tris HCl, 100mM Sodium chloride, pH 8

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

General Info

  • Alternative names
    • p110 BETA
    • p85 alpha
    • p85a
    • PI3 kinase p110 subunit beta
    • PI3 kinase p85 subunit alpha
    • PI3K
    • PI3Kbeta
    • PI3KCB
    • PIK3R1
    • PtdIns 3 kinase p110
    • PtdIns 3 kinase p85 alpha
    see all
  • RelevanceThe enzyme phosphatidylinositol 3 kinase (PI3 kinase) is a lipid kinase that generates phosphatidylinositol 3, 4, 5-triphosphate in response to receptor activation in many signal transduction pathways. Class IA PI3Ks exist as a heterodimer of a catalytic 110 kDa (p110) and a regulatory p85 subunit (p85).
  • Cellular localizationCytoplasmic

Recombinant human PIK3CB + PIK3R1 protein images

  • Lane 1: ab80378 on 10% SDS-PAGE, Coomassie staining, 2µg.
    Lane 2: Protein marker.

References for Recombinant human PIK3CB + PIK3R1 protein (ab80378)

ab80378 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"