Recombinant human PIM1 protein (ab60835)

Overview

Description

  • NatureRecombinant
  • SourceBaculovirus infected Sf9 cells
  • Amino Acid Sequence
    • SpeciesHuman

Associated products

Specifications

Our Abpromise guarantee covers the use of ab60835 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Functional Studies

    SDS-PAGE

  • Purity> 90 % SDS-PAGE.
    Purity: >90% as determined by densitometry.
  • FormLiquid
  • Additional notes

    ab204880 (RPS6 peptide) can be utilized as a substrate for assessing kinase activity

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    Preservative: None
    Constituents: 25% Glycerol, 50mM Tris HCl, 150mM Sodium chloride, 0.25mM DTT, 0.1mM EGTA, 0.1mM EDTA, 0.1mM PMSF, pH 7.5

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

General Info

  • Alternative names
    • Oncogene PIM 1
    • Oncogene PIM1
    • PIM
    • PIM 1
    • Pim 1 kinase
    • pim 1 kinase 44 kDa isoform
    • Pim 1 oncogene
    • pim 1 oncogene (proviral integration site 1)
    • PIM1
    • pim1 kinase 44 kDa isoform
    • PIM1_HUMAN
    • Pim2
    • PIM3
    • Proto oncogene serine/threonine protein kinase Pim 1
    • Proto-oncogene serine/threonine-protein kinase Pim-1
    • Proviral integration site 1
    • Proviral integration site 2
    see all
  • FunctionMay affect the structure or silencing of chromatin by phosphorylating HP1 gamma/CBX3. Isoform 2 promotes the G1/S transition of the cell cycle via up-regulation of CDK2 activity and phosphorylation of CDKN1B, resulting in enhanced nuclear export and proteasome-dependent degradation of CDKN1B. Isoform 2 also represses CDKN1B transcription by phosphorylating and inactivating the transcription factor FOXO3. Plays a role in signal transduction in blood cells. Contributes to both cell proliferation and survival and thus provides a selective advantage in tumorigenesis.
  • Tissue specificityExpressed primarily in cells of the hematopoietic and germline lineages. Isoform 1 and isoform 2 are both expressed in prostate cancer cell lines.
  • Sequence similaritiesBelongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. PIM subfamily.
    Contains 1 protein kinase domain.
  • Post-translational
    modifications
    Autophosphorylated on both serine/threonine and tyrosine residues.
  • Cellular localizationCytoplasm. Nucleus and Cell membrane.
  • Information by UniProt

Recombinant human PIM1 protein images

  • Sample Kinase Activity Plot.
  • ab60835 on SDS-PAGE, MW ~62kDa.

References for Recombinant human PIM1 protein (ab60835)

ab60835 has not yet been referenced specifically in any publications.

Product Wall

Thank you for contacting us. Here are my answers to your questions. Is there a loading limit (cell number) for sample buffer as the lysis buffer? We recommend 1 ml of lysis buffer per 10 million cells. For sample buffer, if you mean gel loading b...

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Thank you for your call yestserday and for your patience while I have looked into your enquiry. This protein has not been pre-boiled, so to run a standard reduced, denatured Western blot, you can add the PIM1 protein to loading buffer such as Laemmli a...

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The Pim1 antibodies we have in our catalog are : ab54457 : the epitope recognized by this antibody maps to a region between aa 250-313 and is specific to Pim-1. No cross-reactivity with Pim-2 or -3 is expected. ab74047 and ab74118 can't react with ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"