Recombinant human PKA beta (catalytic subunit) protein (ab60837)
Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: > 90% SDS-PAGE
- Active: Yes
- Tags: GST tag N-Terminus
- Suitable for: Functional Studies, SDS-PAGE, WB
Description
-
Product name
Recombinant human PKA beta (catalytic subunit) protein
See all PKA beta (catalytic subunit) proteins and peptides -
Purity
> 90 % SDS-PAGE.
Purity: >90% as determined by densitometry. Affinity purified. -
Expression system
Baculovirus infected Sf9 cells -
Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
-
Species
Human -
Predicted molecular weight
65 kDa including tags -
Tags
GST tag N-Terminus
-
Associated products
-
Affibody® Molecule
-
Related Products
-
Substrate reagent
Specifications
Our Abpromise guarantee covers the use of ab60837 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
-
Applications
Functional Studies
SDS-PAGE
Western blot
-
Form
Liquid -
Additional notes
ab204856 (CREB peptide) can be utilized as a substrate for assessing kinase activity
-
Concentration information loading...
Preparation and Storage
-
Stability and Storage
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
pH: 7.50
Constituents: 0.0038% EGTA, 0.00174% PMSF, 0.00385% DTT, 0.79% Tris HCl, 0.00292% EDTA, 25% Glycerol (glycerin, glycerine), 0.87% Sodium chlorideThis product is an active protein and may elicit a biological response in vivo, handle with caution.
General Info
-
Alternative names
- cAMP-dependent protein kinase catalytic beta subunit isoform 4ab
- cAMP-dependent protein kinase catalytic subunit beta
- KAPCB_HUMAN
see all -
Function
Mediates cAMP-dependent signaling triggered by receptor binding to GPCRs. PKA activation regulates diverse cellular processes such as cell proliferation, the cell cycle, differentiation and regulation of microtubule dynamics, chromatin condensation and decondensation, nuclear envelope disassembly and reassembly, as well as regulation of intracellular transport mechanisms and ion flux. -
Tissue specificity
Isoform 1 is most abundant in the brain, with low level expression in kidney. Isoform 2 is predominantly expressed in thymus, spleen and kidney. Isoform 3 and isoform 4 are only expressed in the brain. -
Sequence similarities
Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. cAMP subfamily.
Contains 1 AGC-kinase C-terminal domain.
Contains 1 protein kinase domain. -
Post-translational
modificationsAsn-3 is partially deaminated to Asp giving rise to 2 major isoelectric variants, called CB and CA respectively. -
Cellular localization
Cytoplasm. Nucleus. Translocates into the nucleus (monomeric catalytic subunit) (By similarity). The inactive holoenzyme is found in the cytoplasm. - Information by UniProt
Images
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
-
SDS download
-
Datasheet download
References (0)
ab60837 has not yet been referenced specifically in any publications.