Recombinant human PKC gamma protein (ab60842)

Overview

Description

  • NatureRecombinant
  • SourceBaculovirus infected Sf9 cells
  • Amino Acid Sequence
    • SpeciesHuman
    • TagsGST tag N-Terminus

Specifications

Our Abpromise guarantee covers the use of ab60842 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Functional Studies

    SDS-PAGE

  • FormLiquid
  • Additional notes

    ab204875 (PKC epsilon peptide) can be utilized as a substrate for assessing kinase activity

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    Preservative: None
    Constituents: 25% Glycerol, 50mM Tris HCl, 150mM Sodium chloride, 0.25mM DTT, 0.1mM EGTA, 0.1mM EDTA, 0.1mM PMSF, pH 7.5

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

General Info

  • Alternative names
    • KPCG_HUMAN
    • MGC57564
    • OTTHUMP00000067291
    • PKC-gamma
    • PKCC
    • PKCG
    • PRKCG
    • Protein kinase C gamma
    • Protein kinase C gamma polypeptide
    • Protein kinase C gamma type
    • Protein kinase C, gamma
    • SCA 14
    • SCA14
    see all
  • FunctionThis is a calcium-activated, phospholipid-dependent, serine- and threonine-specific enzyme.
    PKC is activated by diacylglycerol which in turn phosphorylates a range of cellular proteins. PKC also serves as the receptor for phorbol esters, a class of tumor promoters.
  • Tissue specificityExpressed in Purkinje cells of the cerebellar cortex.
  • Involvement in diseaseDefects in PRKCG are the cause of spinocerebellar ataxia type 14 (SCA14) [MIM:605361]. Spinocerebellar ataxia is a clinically and genetically heterogeneous group of cerebellar disorders. Patients show progressive incoordination of gait and often poor coordination of hands, speech and eye movements, due to degeneration of the cerebellum with variable involvement of the brainstem and spinal cord. SCA14 is an autosomal dominant cerebellar ataxia (ADCA).
  • Sequence similaritiesBelongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily.
    Contains 1 AGC-kinase C-terminal domain.
    Contains 1 C2 domain.
    Contains 2 phorbol-ester/DAG-type zinc fingers.
    Contains 1 protein kinase domain.
  • Information by UniProt

Recombinant human PKC gamma protein images

  • Sample Kinase Activity Plot.
  • ab60842 on SDS-PAGE, MW ~105 kDa.

References for Recombinant human PKC gamma protein (ab60842)

ab60842 has not yet been referenced specifically in any publications.

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