Overview

Description

  • Nature
    Recombinant
  • Source
    Baculovirus infected Sf9 cells
  • Amino Acid Sequence
    • Species
      Human

Specifications

Our Abpromise guarantee covers the use of ab60873 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    SDS-PAGE

    Functional Studies

    Western blot

  • Form
    Liquid
  • Additional notes

    ab204856 (CREB peptide) can be utilized as a substrate for assessing kinase activity

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    Preservative: None
    Constituents: 25% Glycerol, 50mM Tris HCl, 150mM Sodium chloride, 0.25mM DTT, 0.1mM EGTA, 0.1mM EDTA, 0.1mM PMSF, pH 7.5

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

General Info

  • Alternative names
    • KPCD1_HUMAN
    • nPKC D1
    • nPKC mu
    • nPKC-D1
    • nPKC-mu
    • nPKCD1
    • nPKCmu
    • PKC
    • PKC MU
    • PKCM
    • PKCmu
    • PKD
    • PKD 1
    • PKD1
    • PRKCM
    • PRKD 1
    • Prkd1
    • Protein kinase C mu
    • Protein kinase C mu type
    • Protein kinase D
    • Protein kinase D1
    • Serine/threonine protein kinase D1
    • Serine/threonine-protein kinase D1
    see all
  • Function
    Converts transient diacylglycerol (DAG) signals into prolonged physiological effects, downstream of PKC. Involved in resistance to oxidative stress through activation of NF-kappa-B.
  • Sequence similarities
    Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. PKD subfamily.
    Contains 1 PH domain.
    Contains 2 phorbol-ester/DAG-type zinc fingers.
    Contains 1 protein kinase domain.
  • Cellular localization
    Cytoplasm. Membrane. Translocation to the cell membrane is required for kinase activation.
  • Information by UniProt

Images

  • Anti-PKC mu antibody (ab5283) at 1/500 dilution + Recombinant human PKC mu protein (ab60873) at 0.1 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Exposure time : 90 seconds
  • Sample Kinase Activity Plot.
  • ab60873 on SDS-PAGE, MW ~131 kDa.

References

ab60873 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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