Recombinant human PKC zeta protein (ab60848)

Overview

Description

  • NatureRecombinant
  • SourceBaculovirus infected Sf9 cells
  • Amino Acid Sequence
    • SpeciesHuman
    • TagsGST tag N-Terminus

Specifications

Our Abpromise guarantee covers the use of ab60848 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Functional Studies

    SDS-PAGE

    Western blot

  • Purity> 90 % SDS-PAGE.
    Purity: >90% as determined by densitometry.
  • FormLiquid
  • Additional notes

    ab204856 (CREB peptide) can be utilized as a substrate for assessing kinase activity

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    Preservative: None
    Constituents: 25% Glycerol, 50mM Tris HCl, 150mM Sodium chloride, 0.25mM DTT, 0.1mM EGTA, 0.1mM EDTA, 0.1mM PMSF, pH 7.5

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

General Info

  • Alternative names
    • 14-3-3-zetaisoform
    • AI098070
    • aPKCzeta
    • C80388
    • EC 2.7.11.13
    • KPCZ_HUMAN
    • nPKC zeta
    • nPKC-zeta
    • OTTHUMP00000001368
    • OTTHUMP00000044160
    • PKC 2
    • PKC ZETA
    • PKC2
    • Pkcz
    • PKCZETA
    • PKM-zeta, included
    • PRKCZ
    • Protein kinase C zeta
    • Protein kinase C zeta form
    • Protein kinase C zeta type
    • r14-3-3
    • R74924
    • zetaPKC
    see all
  • FunctionThis is a calcium-independent, phospholipid-dependent, serine- and threonine-specific enzyme. Phosphorylates 'Ser-311' of RELA subunit of NF-kappa-B.
    PKC is activated by diacylglycerol which in turn phosphorylates a range of cellular proteins. PKC also serves as the receptor for phorbol esters, a class of tumor promoters. Subunit of a quaternary complex that plays a central role in epithelial cell polarization.
  • Tissue specificityExpressed in brain, and to a lesser extent in lung, kidney and testis.
  • Sequence similaritiesBelongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily.
    Contains 1 AGC-kinase C-terminal domain.
    Contains 1 OPR domain.
    Contains 1 phorbol-ester/DAG-type zinc finger.
    Contains 1 protein kinase domain.
  • DomainThe OPR domain mediates mutually exclusive interactions with SQSTM1 and PARD6B.
    The C1 domain does not bind the diacylglycerol (DAG).
  • Post-translational
    modifications
    CDH5 is required for its phosphorylation at Thr-410.
  • Cellular localizationCytoplasm. Endosome. Cell junction. In the retina, localizes in the terminals of the rod bipolar cells (By similarity). Associates with endosomes. Presence of KRIT1, CDH5 and RAP1B is required for its localization to the cell junction.
  • Information by UniProt

Recombinant human PKC zeta protein images

  • Sample Kinase Activity Plot.
  • ab60848 on SDS-PAGE, MW ~93 kDa.
  • Anti-PKC zeta antibody (ab51157) at 1/1000 dilution + Recombinant human PKC zeta protein (ab60848) at 0.01 µg

    Secondary
    Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Exposure time : 1 minute

References for Recombinant human PKC zeta protein (ab60848)

ab60848 has not yet been referenced specifically in any publications.

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