Recombinant human PKN beta protein (ab125615)

Overview

Description

  • NatureRecombinant
  • SourceBaculovirus infected Sf9 cells
  • Amino Acid Sequence
    • AccessionQ6P5Z2
    • SpeciesHuman
    • Molecular weight130 kDa including tags
    • Amino acids1 to 889
    • TagsGST tag N-Terminus

Associated products

Specifications

Our Abpromise guarantee covers the use of ab125615 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Biological activityThe specific activity of ab125615 was determined to be 3 nmol/mg/min.
  • Applications

    Western blot

    Functional Studies

    SDS-PAGE

  • FormLiquid
  • Additional notes

    ab204879 (RPS6 peptide) can be utilized as a substrate for assessing kinase activity

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    pH: 7.50
    Constituents: 0.31% Glutathione, 0.002% PMSF, 0.004% DTT, 0.79% Tris HCl, 0.003% EDTA, 25% Glycerol, 0.88% Sodium chloride

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

General Info

  • Alternative names
    • pkn3
    • PKN3_HUMAN
    • PKNBETA
    • Protein kinase C related kinase 3
    • Protein kinase N3
    • Protein kinase PKN beta
    • Protein kinase PKN-beta
    • Protein-kinase C-related kinase 3
    • RP11-545E17.1
    • Serine/threonine protein kinase N3
    • Serine/threonine-protein kinase N3
    see all
  • FunctionContributes to invasiveness in malignant prostate cancer.
  • Tissue specificityExpressed in prostate tumors and various cancer cell lines. Not expressed in adult tissues.
  • Sequence similaritiesBelongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily.
    Contains 1 AGC-kinase C-terminal domain.
    Contains 1 protein kinase domain.
    Contains 3 REM (Hr1) repeats.
  • DomainThe C1 domain does not bind the diacylglycerol (DAG).
  • Post-translational
    modifications
    Autophosphorylated.
  • Cellular localizationNucleus. Cytoplasm > perinuclear region. Nuclear and perinuclear Golgi region.
  • Information by UniProt

Recombinant human PKN beta protein images

  • Sample Kinase Assay showing the specific activity of ab125615 as 3 nmol/mg/min.
  • SDS-PAGE analysis of ab125615.

References for Recombinant human PKN beta protein (ab125615)

ab125615 has not yet been referenced specifically in any publications.

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I regret to inform you that the immunogen sequence homology between rat and human is only 22% so this antibody should not be cross reacting with rat protein. I apologize for an inconvenience.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"