Overview

Description

  • NatureRecombinant
  • SourceBaculovirus infected Sf9 cells
  • Amino Acid Sequence
    • SpeciesHuman

Specifications

Our Abpromise guarantee covers the use of ab89860 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Biological activityThe specific activity of ab89860 was determined to be 74 nmol/min/mg
  • Applications

    SDS-PAGE

    Western blot

    Functional Studies

  • FormLiquid
  • Additional notes

    ab204856 (CREB peptide) can be utilized as a substrate for assessing kinase activity

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    Preservative: None
    Constituents: 25% Glycerol, 50mM Tris HCl, 150mM Sodium chloride, 0.25mM DTT, 0.1mM EGTA, 0.1mM EDTA, 0.1mM PMSF, pH 7.5

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

General Info

  • Alternative names
    • Cardiolipin activated protein kinase Pak2
    • MGC150606
    • MGC71074
    • PAK 2
    • PAK2
    • PKN gamma
    • PKN2
    • PKN2_HUMAN
    • PRK2
    • PRKCL2
    • PRO2042
    • Protein kinase C like 2
    • Protein kinase C related kinase 2
    • Protein kinase C-like 2
    • Protein kinase N2
    • Protein-kinase C-related kinase 2
    • Serine/threonine-protein kinase N2
    see all
  • FunctionExhibits a preference for highly basic protein substrates.
  • Sequence similaritiesBelongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily.
    Contains 1 AGC-kinase C-terminal domain.
    Contains 1 C2 domain.
    Contains 1 protein kinase domain.
    Contains 3 REM (Hr1) repeats.
  • DomainThe C1 domain does not bind the diacylglycerol (DAG).
  • Post-translational
    modifications
    Autophosphorylated.
    Activated by limited proteolysis with trypsin.
  • Cellular localizationCytoplasm.
  • Information by UniProt

Recombinant human PKN2 protein images

  • Kinase Assay demonstrating specific activity of ab89860
  • SDS-PAGE showing ab89860 at approximately 145kDa

References for Recombinant human PKN2 protein (ab89860)

ab89860 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"