Recombinant Human PLA2G1B protein (ab63842)

Overview

Description

  • NatureRecombinant
  • SourceEscherichia coli
  • Amino Acid Sequence
    • AccessionP04054
    • SpeciesHuman
    • SequenceMRGSHHHHHH GMASHMAVWQ FRKMIKCVIP GSDPFLEYNN YGCYCGLGGS GTPVDELDKC CQTHDNCYDQ AKKLDSCKFL LDNPYTHTYS YSCSGSAITC SSKNKECEAF ICNCDRNAAI CFSKAPYNKA HKNLDTKKYC QS
    • Amino acids23 to 148
    • TagsHis tag N-Terminus
    • Additional sequence informationTotal 142 AA. MW: 16 kDa (calculated). N-Terminal His-tag, 16 extra AA

Specifications

Our Abpromise guarantee covers the use of ab63842 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Western blot

  • Purity> 95 % SDS-PAGE.

  • FormLyophilised
  • Additional notesProduct is NOT sterile! Please filter the product by an appropriate sterile filter before using it in cell culture.
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.

    Preservative: None
    0.05M Acetate buffer pH 4.0

  • ReconstitutionAdd 0.1M Acetate buffer pH4 to prepare a working stock solution of approximately 0.5 mg/ml and let the lyophilized pellet dissolve completely. For conversion into higher pH value, we recommend intensive dilution by relevant buffer to a concentration of 10µg/ml. In higher concentrations the solubility of this antigen is limited.

General Info

  • Alternative names
    • Group IB phospholipase A2
    • Group IB secretory phospholipase A2
    • MGC119834
    • MGC119835
    • PA21B_HUMAN
    • Phosphatidylcholine 2 acylhydrolase
    • Phosphatidylcholine 2-acylhydrolase 1B
    • Phospholipase A2
    • Phospholipase A2 group IB
    • Phospholipase A2 group IB (pancreas)
    • PLA 2
    • PLA2
    • PLA2A
    • PLA2G1B
    • PPLA 2
    • PPLA2
    • sPLA2IB
    see all
  • FunctionPA2 catalyzes the calcium-dependent hydrolysis of the 2-acyl groups in 3-sn-phosphoglycerides.
  • Sequence similaritiesBelongs to the phospholipase A2 family.
  • Cellular localizationSecreted.
  • Information by UniProt

Recombinant Human PLA2G1B protein images

  • 12% SDS-PAGE separation of ab63842. Molecular Weight: ~16 kDa.

    Lane 1. Molecular weight marker
    Lane 2. reduced and heated sample, 5µg/lane
    Lane 3. non-reduced and non-heated sample, 5µg/lane

References for Recombinant Human PLA2G1B protein (ab63842)

ab63842 has not yet been referenced specifically in any publications.

Product Wall

Thank you for providing that information and sorry for the delay in getting back to you.
The information provided has been very useful and I will look into whether these antibodies would be likely to detect the protein and what may be contributing...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"