Recombinant Human PLA2G1B protein (ab63842)

Overview

Description

  • Nature
    Recombinant
  • Source
    Escherichia coli
  • Amino Acid Sequence
    • Accession
    • Species
      Human
    • Sequence
      MRGSHHHHHH GMASHMAVWQ FRKMIKCVIP GSDPFLEYNN YGCYCGLGGS GTPVDELDKC CQTHDNCYDQ AKKLDSCKFL LDNPYTHTYS YSCSGSAITC SSKNKECEAF ICNCDRNAAI CFSKAPYNKA HKNLDTKKYC QS
    • Amino acids
      23 to 148
    • Tags
      His tag N-Terminus
    • Additional sequence information
      Total 142 AA. MW: 16 kDa (calculated). N-Terminal His-tag, 16 extra AA

Specifications

Our Abpromise guarantee covers the use of ab63842 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Western blot

  • Purity
    > 95 % SDS-PAGE.

  • Form
    Lyophilised
  • Additional notes
    Product is NOT sterile! Please filter the product by an appropriate sterile filter before using it in cell culture.
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.

    Preservative: None
    0.05M Acetate buffer pH 4.0

  • Reconstitution
    Add 0.1M Acetate buffer pH4 to prepare a working stock solution of approximately 0.5 mg/ml and let the lyophilized pellet dissolve completely. For conversion into higher pH value, we recommend intensive dilution by relevant buffer to a concentration of 10µg/ml. In higher concentrations the solubility of this antigen is limited.

General Info

  • Alternative names
    • Group IB phospholipase A2
    • Group IB secretory phospholipase A2
    • MGC119834
    • MGC119835
    • PA21B_HUMAN
    • Phosphatidylcholine 2 acylhydrolase
    • Phosphatidylcholine 2-acylhydrolase 1B
    • Phospholipase A2
    • Phospholipase A2 group IB
    • Phospholipase A2 group IB (pancreas)
    • PLA 2
    • PLA2
    • PLA2A
    • PLA2G1B
    • PPLA 2
    • PPLA2
    • sPLA2IB
    see all
  • Function
    PA2 catalyzes the calcium-dependent hydrolysis of the 2-acyl groups in 3-sn-phosphoglycerides.
  • Sequence similarities
    Belongs to the phospholipase A2 family.
  • Cellular localization
    Secreted.
  • Information by UniProt

Images

  • 12% SDS-PAGE separation of ab63842. Molecular Weight: ~16 kDa.

    Lane 1. Molecular weight marker
    Lane 2. reduced and heated sample, 5µg/lane
    Lane 3. non-reduced and non-heated sample, 5µg/lane

References

ab63842 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Thank you for providing that information and sorry for the delay in getting back to you.
The information provided has been very useful and I will look into whether these antibodies would be likely to detect the protein and what may be contributing...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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