The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
% by SDS-PAGE.
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Preparation and Storage
Stability and Storage
Shipped at 4°C. After reconstitution store at -20ºC. Avoid freeze / thaw cycles.
Constituents: 10% Trehalose, 1% Human serum albumin
ReconstitutionIt is recommended that 0.5 ml of sterile phosphate-buffered saline be added to the vial.
Following reconstitution short-term storage at 4°C is recommended, with longer-term storage in aliquots at -18 to -20°C. Repeated freeze thawing is not recommended.
Placenta growth factor
Placental growth factor
Placental growth factor, vascular endothelial growth factor related protein
FunctionGrowth factor active in angiogenesis and endothelial cell growth, stimulating their proliferation and migration. It binds to the receptor FLT1/VEGFR-1. Isoform PlGF-2 binds NRP1/neuropilin-1 and NRP2/neuropilin-2 in a heparin-dependent manner.
Tissue specificityWhile the three isoforms are present in most placental tissues, PlGF-2 is specific to early (8 week) placenta and only PlGF-1 is found in the colon and mammary carcinomas.
Sequence similaritiesBelongs to the PDGF/VEGF growth factor family.
DomainIsoform PlGF-2 contains a basic insert which acts as a cell retention signal.
Cellular localizationSecreted. The three isoforms are secreted but PlGF-2 appears to remain cell attached unless released by heparin.
Densitometry of protein isoforms visualised by 2-DE.
The densitometry scan demonstrates the purified human cell expressed protein exists in multiple isoforms, which differ according to their level of post-translational
The triangle indicates the theoretical MW and pI of the protein.
SDS-PAGE - PIGF protein (ab84148)
1D SDS-PAGE of ab84148 before and after treatment with glycosidases to remove oligosaccharides.
Lane 1: ab84148
Lane 2: ab84148 treated with PNGase F to remove potential N-linked glycans
Lane 3: ab84148 treated with a glycosidase cocktail to remove potential N- and O-linked glycans.
10µg protein loaded per lane; Deep Purple™ stained.
Drop in MW after treatment with PNGase F indicates presence
of N-linked glycans. A further drop in MW after treatment with the glycosidase cocktail indicates the presence of O-linked glycans. Additional bands in lane 2 and lane 3 are glycosidase enzymes.
References for Recombinant Human PLGF protein (ab84148)
has not yet been referenced specifically in any publications.
Publishing research using ab84148? Please let us know so that we can cite the reference in this datasheet.
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