Overview

Description

  • NatureRecombinant
  • SourceBaculovirus infected Sf9 cells
  • Amino Acid Sequence
    • AccessionQ9H4B4
    • SpeciesHuman
    • Molecular weight60 kDa including tags
    • Amino acids57 to 340

Associated products

Specifications

Our Abpromise guarantee covers the use of ab125627 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Biological activityThe specific activity of PLK3 was determined to be 2.2 nmol/min/mg.
  • Applications

    Western blot

    Functional Studies

    SDS-PAGE

  • FormLiquid
  • Additional notes

    ab91090 (Cow Casein full length protein) can be utilized as a substrate for assessing Kinase activity

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    pH: 7.50
    Constituents: 0.31% Glutathione, 0.002% PMSF, 0.004% DTT, 0.79% Tris HCl, 0.003% EDTA, 25% Glycerol, 0.88% Sodium chloride

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

General Info

  • Alternative names
    • CNK
    • Cytokine Inducible Kinase
    • Cytokine-inducible serine/threonine-protein kinase
    • FGF inducible kinase
    • FGF-inducible kinase
    • FNK
    • PLK-3
    • Plk3
    • PLK3_HUMAN
    • Polo Like Kinase 3
    • Polo-like kinase 3
    • PRK
    • Proliferation Related Kinase
    • Proliferation-related kinase
    • Serine/threonine-protein kinase PLK3
    see all
  • FunctionSerine/threonine protein kinase involved in regulating M phase functions during the cell cycle. May also be part of the signaling network controlling cellular adhesion. In vitro, is able to phosphorylate CDC25C and casein.
  • Tissue specificityTranscripts are highly detected in placenta, lung, followed by skeletal muscle, heart, pancreas, ovaries and kidney and weakly detected in liver and brain. May have a short half-live. In cells of hematopoietic origin, strongly and exclusively detected in terminally differentiated macrophages. Transcript expression appears to be down-regulated in primary lung tumor.
  • Sequence similaritiesBelongs to the protein kinase superfamily. Ser/Thr protein kinase family. CDC5/Polo subfamily.
    Contains 2 POLO box domains.
    Contains 1 protein kinase domain.
  • Post-translational
    modifications
    Phosphorylated as cells enter mitosis and dephosphorylated as cells exit mitosis.
  • Cellular localizationMembrane.
  • Information by UniProt

Recombinant human PLK3 protein images

  • SDS-PAGe of Human PLK3 protein.
  • The specific activity of ab125627 was determined to be 2.2 nmol/min/mg.

References for Recombinant human PLK3 protein (ab125627)

ab125627 has not yet been referenced specifically in any publications.

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