• NatureRecombinant
  • SourceBaculovirus infected Sf9 cells
  • Amino Acid Sequence
    • AccessionO00444
    • SpeciesHuman
    • Molecular weight140 kDa including tags
    • Amino acids1 to 836


Our Abpromise guarantee covers the use of ab125558 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Biological activityThe specific activity of ab125558 is 5.6 nmol/min/mg.
  • Applications

    Western blot

    Functional Studies


  • Purity> 70 % Densitometry.
    ab125558 is >70% pure.
  • FormLiquid
  • Additional notes

    ab64311 (Myelin Basic Protein protein) can be utilized as a substrate for assessing kinase activity

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    pH: 7.50
    Constituents: 0.31% Glutathione, 0.002% PMSF, 0.004% DTT, 0.79% Tris HCl, 0.003% EDTA, 25% Glycerol, 0.88% Sodium chloride

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

General Info

  • Alternative names
    • PLK 4
    • PLK-4
    • PLK4
    • PLK4_HUMAN
    • Polo like Kinase 4
    • Polo-like kinase 4
    • Protein serine/threonine kinase
    • SAK
    • Serine/threonine kinase
    • Serine/Threonine Kinase 18
    • Serine/threonine protein kinase 18
    • Serine/threonine protein kinase PLK4
    • Serine/threonine protein kinase Sak
    • Serine/threonine-protein kinase 18
    • Serine/threonine-protein kinase PLK4
    • Serine/threonine-protein kinase SAK
    • Snk Akin Kinase
    • STK 18
    • STK18
    see all
  • FunctionSerine/threonine-protein kinase that plays a central role in centriole duplication. Able to trigger procentriole formation on the surface of the parental centriole cylinder, leading to the recruitment of centriole biogenesis proteins such as SASS6, CENPJ/CPAP, CP110, CEP135 and gamma-tubulin. When overexpressed, it is able to induce centrosome amplification through the simultaneous generation of multiple procentrioles adjoining each parental centriole during S phase. Its central role in centriole replication suggests a posible role in tumorigenesis, centrosome aberrations being frequently observed in tumors. Also involved in trophoblast differentiation by phosphorylating HAND1, leading to disrupt the interaction between HAND1 and MDFIC and activate HAND1. Phosphorylates CDC25C and CHEK2/CHK2.
  • Sequence similaritiesBelongs to the protein kinase superfamily. Ser/Thr protein kinase family. CDC5/Polo subfamily.
    Contains 1 POLO box domain.
    Contains 1 protein kinase domain.
  • Post-translational
    Ubiquitinated; leading to its degradation by the proteasome.
    Tyrosine-phosphorylated by TEC.
  • Cellular localizationCytoplasm > cytoskeleton > centrosome > centriole. Nucleus > nucleolus. Cleavage furrow. Associates with centrioles throughout the cell cycle. According to PubMed:16244668, it is not present at cleavage furrows.
  • Information by UniProt

Recombinant human PLK4 protein images

  • The specific activity of ab125558 was determined to be 5.6 nmol/min/mg.
  • SDS PAGE analysis of ab125558 (PLK4 protein).

References for Recombinant human PLK4 protein (ab125558)

ab125558 has not yet been referenced specifically in any publications.

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