Overview

Description

  • NatureRecombinant
  • SourceEscherichia coli
  • Amino Acid Sequence
    • SpeciesHuman

Associated products

Specifications

Our Abpromise guarantee covers the use of ab53376 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Western blot

    SDS-PAGE

  • Purity> 95 % SDS-PAGE.

  • FormLiquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

    Preservative: None
    Constituents: 50% Glycerol, 20mM Tris, pH 8

General Info

  • Alternative names
    • A esterase 1
    • A-esterase 1
    • Aromatic esterase 1
    • Arylesterase 1
    • Arylesterase B type
    • ESA
    • Esterase A
    • K 45
    • K-45
    • MVCD5
    • Paraoxonase
    • Paraoxonase 1
    • Paraoxonase B type
    • Paraoxonase, plasma
    • Paraoxonase1
    • PON
    • PON 1
    • PON1
    • PON1_HUMAN
    • Serum aryldiakylphosphatase
    • Serum aryldialkylphosphatase 1
    • Serum paraoxonase/arylesterase 1
    see all
  • FunctionHydrolyzes the toxic metabolites of a variety of organophosphorus insecticides. Capable of hydrolyzing a broad spectrum of organophosphate substrates and lactones, and a number of aromatic carboxylic acid esters. Mediates an enzymatic protection of low density lipoproteins against oxidative modification and the consequent series of events leading to atheroma formation.
  • Tissue specificityPlasma, associated with HDL (at protein level). Expressed in liver, but not in heart, brain, placenta, lung, skeletal muscle, kidney or pancreas.
  • Involvement in diseaseGenetic variation in PON1 is associated with susceptibility to microvascular complications of diabetes type 5 (MVCD5) [MIM:612633]. These are pathological conditions that develop in numerous tissues and organs as a consequence of diabetes mellitus. They include diabetic retinopathy, diabetic nephropathy leading to end-stage renal disease, and diabetic neuropathy. Diabetic retinopathy remains the major cause of new-onset blindness among diabetic adults. It is characterized by vascular permeability and increased tissue ischemia and angiogenesis. Note=Homozygosity for the Leu-54 allele is strongly associated with the development of retinal disease in diabetic patients.
  • Sequence similaritiesBelongs to the paraoxonase family.
  • Post-translational
    modifications
    Glycosylated.
    The signal sequence is not cleaved.
    Present in two forms, form B contains a disulfide bond, form A does not.
  • Cellular localizationSecreted > extracellular space.
  • Information by UniProt

Recombinant Human PON1 protein images

  • SDS Page analysis of ab53376.
  • Anti-PON1 antibody [17A12] (ab24261) at 1/500 dilution + Recombinant Human PON1 protein (ab53376) at 0.01 µg

    Secondary
    Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/500 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Exposure time : 1 minute

References for Recombinant Human PON1 protein (ab53376)

ab53376 has not yet been referenced specifically in any publications.

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