The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Biological activitySpecific activity is > 200 nmoles/min/mg, and is defined as the amount of enzyme that cleaves 1 µmole of suc-AAFP-pNA per minute at 25°C in Tris-Hcl pH8.0 using chymotrypsin.
Prepare 170 µl assay buffer into a suitable container and pre-chill on ice before use: The final concentrations are 200 mM Tris-Hcl, pH 8.0, and 20nM chymotrypsin.
Add 1 µg of recombinant Cyclophilin G protein to assay buffer.
Mix by inversion and equilibrate to 1°C and monitor the A405nm until the value is constant using a spectrophotometer.
Add 20 µl pre-chilled 5mM suc-AAFP-pNA. (Substrate was dissolved in TFE that contained 460mM LiCl to a concentration of 3 mM)
Record the increase in A405 nm for 30 minutes at 25°C.
% SDS-PAGE. ab90688 is purified using conventional chromatography techniques.
Concentration information loading...
Preparation and Storage
Stability and Storage
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Clk associating RS cyclophilin
Peptidyl prolyl cis trans isomerase G
Peptidyl prolyl isomerase G
Peptidyl-prolyl cis-trans isomerase G
Peptidyl-prolyl isomerase G
Peptidylprolyl isomerase G
Peptidylprolyl isomerase G (cyclophilin G)
SR related CTD associated factor 10
FunctionPPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides. May be implicated in the folding, transport, and assembly of proteins. May play an important role in the regulation of pre-mRNA splicing.