Overview

Description

  • Nature
    Recombinant
  • Source
    Escherichia coli
  • Amino Acid Sequence
    • Species
      Human
    • Sequence
      MGSSHHHHHH SSGLVPRGSH MGIKVQRPRC FFDIAINNQP AGRVVFELFS DVCPKTCENF RCLCTGEKGT GKSTQKPLHY KSCLFHRVVK DFMVQGGDFS EGNGRGGESI YGGFFEDESF AVKHNKEFLL SMANRGKDTN GSQFFITTKP TPHLDGHHVV FGQVISGQEV VREIENQKTD AASKPFAEVR ILSCG
    • Amino acids
      1 to 175

Specifications

Our Abpromise guarantee covers the use of ab90688 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Biological activity
    Specific activity is > 200 nmoles/min/mg, and is defined as the amount of enzyme that cleaves 1 µmole of suc-AAFP-pNA per minute at 25°C in Tris-Hcl pH8.0 using chymotrypsin.
    Activity Assay
    1. Prepare 170 µl assay buffer into a suitable container and pre-chill on ice before use: The final concentrations are 200 mM Tris-Hcl, pH 8.0, and 20nM chymotrypsin.
    2. Add 1 µg of recombinant Cyclophilin G protein to assay buffer.
    3. Mix by inversion and equilibrate to 1°C and monitor the A405nm until the value is constant using a spectrophotometer.
    4. Add 20 µl pre-chilled 5mM suc-AAFP-pNA. (Substrate was dissolved in TFE that contained 460mM LiCl to a concentration of 3 mM)
    5. Record the increase in A405 nm for 30 minutes at 25°C.
  • Applications

    SDS-PAGE

  • Mass spectrometry
    MALDI-TOF
  • Purity
    > 95 % SDS-PAGE.
    ab90688 is purified using conventional chromatography techniques.
  • Form
    Liquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

    Preservative: None
    Constituents: 10% Glycerol, 20mM Tris HCl, 1mM DTT, pH 8.0

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

General Info

  • Alternative names
    • CARS cyclophilin
    • CARS Cyp
    • CARS-cyclophilin
    • CARS-Cyp
    • CASP 10
    • CASP10
    • Clk associating RS cyclophilin
    • Clk-associating RS-cyclophilin
    • Cyclophilin G
    • CyclophilinG
    • CYP
    • MGC133241
    • Peptidyl prolyl cis trans isomerase G
    • Peptidyl prolyl isomerase G
    • Peptidyl prolyl isomerase G (cyclophilin G)
    • Peptidyl-prolyl cis-trans isomerase G
    • Peptidyl-prolyl isomerase G
    • Peptidylprolyl isomerase G
    • Peptidylprolyl isomerase G (cyclophilin G)
    • PPIase G
    • Ppig
    • PPIG_HUMAN
    • Rotamase G
    • RotamaseG
    • SCAF10
    • SR cyclophilin
    • SR cyp
    • SR related CTD associated factor 10
    • SR-cyclophilin
    • SR-cyp
    • SRcyp
    see all
  • Function
    PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides. May be implicated in the folding, transport, and assembly of proteins. May play an important role in the regulation of pre-mRNA splicing.
  • Tissue specificity
    Ubiquitous.
  • Sequence similarities
    Contains 1 PPIase cyclophilin-type domain.
  • Domain
    The RS domain is required for the interaction with the phosphorylated C-terminal domain of RNA polymerase II.
  • Post-translational
    modifications
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localization
    Nucleus matrix. Nucleus speckle. Colocalizes with RNA splicing factors at nuclear speckles.
  • Information by UniProt

Images

  • 15% SDS-PAGE (3 µg), using ab90688.

References

ab90688 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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