Overview

Description

  • Nature
    Recombinant
  • Source
    Escherichia coli
  • Amino Acid Sequence
    • Species
      Human
    • Sequence
      MAVANSSPVNPVVFFDVSIGGQEVGRMKIELFADVVPKTAENFRQFCTGE FRKDGVPIGYKGSTFHRVIKDFMIQGGDFVNGDGTGVASIYRGPFADENF KLRHSAPGLLSMANSGPSTNGCQFFITCSKCDWLDGKHVVFGKIIDGLLV MRKIENVPTGPNNKPKLPVVISQCGEM

Associated products

Specifications

Our Abpromise guarantee covers the use of ab78874 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Biological activity
    Specific activity is > 220 nmoles/min/mg, and is defined as the amount of enzyme that cleaves 1 µmole of suc-AAFP-pNA per minute at 25°C in Tris-Hcl pH8.0 using chymotrypsin.

    Activity Assay

    1. Prepare 170 µl assay buffer into a suitable container and pre-chill on ice before use: The final concentrations are 200 mM Tris-Hcl, pH 8.0, and 20nM chymotrypsin.
    2. Add 10 µl of recombinant Cyclophikin H (PPIH) protein with 1 µg in assay buffer.
    3. Mix by inversion and equilibrate to 1°C and monitor the A405nm until the value is constant using a spectrophotometer.
    4. Add 20 µl pre-chilled 5mM suc-AAFP-pNA. (Substrate was dissolved in TFE that contained 460mM LiCl to a concentration of 3 mM)
    5. Record the increase in A405 nm for 30 minutes at 25°C.
  • Applications

    SDS-PAGE

    Functional Studies

  • Endotoxin level
    < 1.000 Eu/µg
  • Mass spectrometry
    MALDI-TOF
  • Purity
    > 95 % SDS-PAGE.
    ab78874 is purified using conventional chromatography techniques.
  • Form
    Liquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

    Preservative: None
    Constituents: 10% Glycerol, PBS, pH 7.4

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

General Info

  • Alternative names
    • Cyclophilin H
    • CYP-20
    • CypH
    • Peptidyl prolyl cis trans isomerase H
    • Peptidyl-prolyl cis-trans isomerase H
    • Peptidylprolyl isomerase H
    • PPIase H
    • PPIH
    • PPIH_HUMAN
    • RGD1564921
    • Rotamase H
    • Small nuclear ribonucleoprotein particle specific cyclophilin H
    • Small nuclear ribonucleoprotein particle-specific cyclophilin H
    • SnuCyp 20
    • U snRNP associated cyclophilin SnuCyp 20
    • U-snRNP-associated cyclophilin SnuCyp-20
    • USA CYP
    • USA-CYP
    • USACYP
    see all
  • Function
    PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides. Participates in pre-mRNA splicing. May play a role in the assembly of the U4/U5/U6 tri-snRNP complex. May act as a chaperone.
  • Sequence similarities
    Belongs to the cyclophilin-type PPIase family. PPIase H subfamily.
    Contains 1 PPIase cyclophilin-type domain.
  • Cellular localization
    Nucleus speckle. Cytoplasm. Colocalizes with spliceosomal snRNPs. A small proportion may also be cytoplasmic.
  • Information by UniProt

Images

  • 15% SDS-PAGE showing ab78874 at approximately 19kDa (3µg).

References

ab78874 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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