Overview

Description

  • Nature
    Recombinant
  • Source
    Escherichia coli
  • Amino Acid Sequence
    • Species
      Human
    • Sequence
      MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWILMGAF LDKPKMEKHN AQGQGNGLRY GLSSMQGWRV EMEDAHTAVI GLPSGLESWS FFAVYDGHAG SQVAKYCCEH LLDHITNNQD FKGSAGAPSV ENVKNGIRTG FLEIDEHMRV MSEKKHGADR SGSTAVGVLI SPQHTYFINC GDSRGLLCRN RKVHFFTQDH KPSNPLEKER IQNAGGSVMI QRVNGSLAVS RALGDFDYKC VHGKGPTEQL VSPEPEVHDI ERSEEDDQFI ILACDGIWDV MGNEELCDFV RSRLEVTDDL EKVCNEVVDT CLYKGSRDNM SVILICFPNA PKVSPEAVKK EAELDKYLEC RVEEIIKKQG EGVPDLVHVM RTLASENIPS LPPGGELASK RNVIEAVYNR LNPYKNDDTD STSTDDMW
    • Amino acids
      1 to 382

Associated products

Specifications

Our Abpromise guarantee covers the use of ab51205 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Biological activity
    Specific activity: > 8,000units/mg of PPM1A. Unit Definition - one unit will hydrolyze 1 nanomole of p-nitrophenylphosphatate per minute at pH 7.4 at 37°C using 10mM of substrate.
  • Applications

    SDS-PAGE

    Functional Studies

  • Endotoxin level
    < 1.000 Eu/µg
  • Mass spectrometry
    MALDI-TOF
  • Purity
    > 95 % SDS-PAGE.
    ab51205 was purified by conventional column chromatography.
  • Form
    Liquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

    Preservative: None
    Constituents: 20% Glycerol, 50mM Sodium chloride, 2mM DTT, 10mM Tris HCl, 1mM Manganese chloride, pH 7.5

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

General Info

  • Alternative names
    • Protein phosphatase 2C isoform alpha
    • EC 3.1.3.16
    • FLJ42306
    • IA
    • MGC9201
    • Mpp alpha
    • PP2C alpha
    • PP2C-alpha
    • PP2CA
    • PP2Calpha
    • PPM 1A
    • PPM1A
    • PPM1A_HUMAN
    • PPPM1A
    • Protein phosphatase 1A
    • Protein phosphatase 1A (formerly 2C) magnesium dependent alpha isoform
    • Protein phosphatase 1A magnesium dependent alpha
    • Protein phosphatase 2C alpha
    • Protein phosphatase 2C alpha isoform
    • Protein phosphatase 2C isoform alpha
    • Protein phosphatase IA
    • Protein phosphatase, Mg2+/Mn2+ dependent, 1A
    see all
  • Function
    Enzyme with a broad specificity. Negatively regulates TGF-beta signaling through dephosphorylating SMAD2 and SMAD3, resulting in their dissociation from SMAD4, nuclear export of the SMADs and termination of the TGF-beta-mediated signaling.
  • Sequence similarities
    Belongs to the PP2C family.
  • Cellular localization
    Nucleus.
  • Information by UniProt

Images

  • 10% SDS-PAGE gel loaded with ab51205, PP2C alpha protein (His tag).
    Predicted molecular weight 46.6 kDa.

References

ab51205 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab51205.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up