Overview

Description

  • Nature
    Recombinant
  • Source
    Baculovirus infected Sf9 cells
  • Amino Acid Sequence
    • Accession
    • Species
      Human
    • Molecular weight
      79 kDa including tags
    • Amino acids
      1 to 471

Associated products

Specifications

Our Abpromise guarantee covers the use of ab125743 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Biological activity
    The specific activity of ab125743 was determined to be 172 nmol/min/mg.
  • Applications

    Western blot

    Functional Studies

    SDS-PAGE

  • Purity
    > 80 % Densitometry.
    Purity was determined to be >80% by densitometry.
  • Form
    Liquid
  • Additional notes

    ab204861 (Hsp27 peptide) can be utilized as a substrate for assessing kinase activity

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    pH: 7.50
    Constituents: 0.31% Glutathione, 0.002% PMSF, 0.004% DTT, 0.79% Tris HCl, 0.003% EDTA, 25% Glycerol, 0.88% Sodium chloride

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

General Info

  • Alternative names
    • MAP kinase-activated protein kinase 5
    • MAPK-activated protein kinase 5
    • MAPK5_HUMAN
    • MAPKAP kinase 5
    • MAPKAPK-5
    • MAPKAPK5
    • Mitogen activated protein kinase activated protein kinase 5
    • p38-regulated/activated protein kinase
    • PRAK
    see all
  • Function
    Mediates stress-induced small heat shock protein 27 phosphorylation.
  • Tissue specificity
    Expressed ubiquitously.
  • Sequence similarities
    Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family.
    Contains 1 protein kinase domain.
  • Post-translational
    modifications
    Phosphorylated on Thr-182; which is the regulatory phosphorylation site and is located on the T-loop/loop 12.
  • Cellular localization
    Cytoplasm. Nucleus. Also observed in the nucleus.
  • Information by UniProt

Recombinant human PRAK protein images

  • SDS-PAGE analysis of ab125743.
  • Sample Kinase Assay showing the specific activity of ab125743 to be 172 nmol/min/mg.

References for Recombinant human PRAK protein (ab125743)

ab125743 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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