Recombinant Human proBNP protein (ab51403)

Overview

Description

  • NatureRecombinant
  • SourceEscherichia coli
  • Amino Acid Sequence
    • SpeciesHuman
    • SequenceHPLG SPGSASDLET SGLQEQRNHL QGKLSELQVE QTSLEPLQES PRPTGVWKSR EVATEGIRGH RKMVLYTLRA PR
    • Amino acids27 to 102
    • TagsHis tag N-Terminus

Specifications

Our Abpromise guarantee covers the use of ab51403 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Biological activityab51403 corresponds to the first 76 aa residues after the signal peptide has been cleved.
  • Applications

    Western blot

  • Purity> 90 % SDS-PAGE.
    ab51403 was purified by a combination of affinity purification and ion exchange chromatography
  • FormLiquid
  • Additional notesab51403 corresponds to the first 76 aa residues after the signal peptide has been cleved.
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

General Info

  • Alternative names
    • ANFB_HUMAN
    • BNP
    • BNP(1-32)
    • BNP(5-29)
    • BNP-32
    • Brain type natriuretic peptide
    • Gamma brain natriuretic peptide
    • Gamma-brain natriuretic peptide
    • Natriuretic peptide precursor B
    • nppb
    • NT proBNP
    see all
  • FunctionCardiac hormone which may function as a paracrine antifibrotic factor in the heart. Also plays a key role in cardiovascular homeostasis through natriuresis, diuresis, vasorelaxation, and inhibition of renin and aldosterone secretion. Specifically binds and stimulates the cGMP production of the NPR1 receptor. Binds the clearance receptor NPR3.
  • Tissue specificityBrain and also in atria, but at much lower levels than ANP.
  • Sequence similaritiesBelongs to the natriuretic peptide family.
  • Cellular localizationSecreted.
  • Information by UniProt

References for Recombinant Human proBNP protein (ab51403)

ab51403 has not yet been referenced specifically in any publications.

Product Wall

This protein has a 6 His tag on the N terminus.

To answer your questions:

1) MW = 8.46 kDa (with His-tag: 9.28 kDa)
ab51403 corresponds to the first 76 aa residues after the signal peptide has been cleaved, not the full length unprocessed precursor whose MW is about 15 kDa (http://www....

Read More

Thank you for your reply. There are two amino acids "LE" before the 6x His tag. Please let me know if you need any additional information.

Thank you for your patience. My colleague has confirmed thatthere were amino acids that were added before the His tag for ab51403. I hope this helps, please let me know if you need any additional information or assistance.

Thank you for contacting us. The His tag is at C-terminal end of protein. I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"