Recombinant Human Profilin 1 protein (ab87760)
Key features and details
- Expression system: Escherichia coli
- Purity: > 95% SDS-PAGE
- Suitable for: SDS-PAGE
Description
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Product name
Recombinant Human Profilin 1 protein
See all Profilin 1 proteins and peptides -
Purity
> 95 % SDS-PAGE.
ab87760 is purified using conventional chromatography techniques. -
Expression system
Escherichia coli -
Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Sequence
MAGWNAYIDN LMADGTCQDA AIVGYKDSPS VWAAVPGKTF VNITPAEVGV LVGKDRSSFY VNGLTLGGQK CSVIRDSLLQ DGEFSMDLRT KSTGGAPTFN VTVTKTDKTL VLLMGKEGVH GGLINKKCYE MASHLRRSQY
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Associated products
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Related Products
Specifications
Our Abpromise guarantee covers the use of ab87760 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
SDS-PAGE
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Form
Liquid -
Additional notes
Endotoxin level: < 1.0 EU per 1 µg of protein (determined by LAL method). -
Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
pH: 8.00
Constituents: 0.316% Tris HCl, 10% Glycerol
General Info
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Alternative names
- Actin binding protein
- ALS18
- Epididymis tissue protein Li 184a
see all -
Function
Binds to actin and affects the structure of the cytoskeleton. At high concentrations, profilin prevents the polymerization of actin, whereas it enhances it at low concentrations. By binding to PIP2, it inhibits the formation of IP3 and DG. -
Sequence similarities
Belongs to the profilin family. -
Cellular localization
Cytoplasm > cytoskeleton. - Information by UniProt
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (6)
ab87760 has been referenced in 6 publications.
- Ramírez de Mingo D et al. Conformational dynamics in the disordered region of human CPEB3 linked to memory consolidation. BMC Biol 20:129 (2022). PubMed: 35658951
- Allen A et al. Actin-binding protein profilin1 promotes aggressiveness of clear-cell renal cell carcinoma cells. J Biol Chem 295:15636-15649 (2020). PubMed: 32883810
- George L et al. Profilin-1 is dysregulated in endometroid (type I) endometrial cancer promoting cell proliferation and inhibiting pro-inflammatory cytokine production. Biochem Biophys Res Commun 531:459-464 (2020). PubMed: 32800551
- Menkhorst EM et al. Invasive trophoblast promote stromal fibroblast decidualization via Profilin 1 and ALOX5. Sci Rep 7:8690 (2017). PubMed: 28821715
- Zienkiewicz A et al. Chip-based capillary electrophoresis profiling of olive pollen extracts used for allergy diagnosis and immunotherapy. Electrophoresis 35:2681-5 (2014). PubMed: 24888349
- Caglayan E et al. Profilin-1 is expressed in human atherosclerotic plaques and induces atherogenic effects on vascular smooth muscle cells. PLoS One 5:e13608 (2010). PubMed: 21049052