The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
% SDS-PAGE. ab87760 is purified using conventional chromatography techniques.
Endotoxin level: < 1.0 EU per 1 µg of protein (determined by LAL method).
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Preparation and Storage
Stability and Storage
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Constituents: 10% Glycerol, 20mM Tris HCl, pH 8.0
Actin binding protein
Epididymis tissue protein Li 184a
Binds to actin and affects the structure of the cytoskeleton. At high concentrations, profilin prevents the polymerization of actin, whereas it enhances it at low concentrations. By binding to PIP2, it inhibits the formation of IP3 and DG.
References for Recombinant Human Profilin 1 protein (ab87760)
This product has been referenced in:
Zienkiewicz A et al. Chip-based capillary electrophoresis profiling of olive pollen extracts used for allergy diagnosis and immunotherapy. Electrophoresis35:2681-5 (2014).
Read more (PubMed: 24888349) »
Caglayan E et al. Profilin-1 is expressed in human atherosclerotic plaques and induces atherogenic effects on vascular smooth muscle cells. PLoS One5:e13608 (2010).
Read more (PubMed: 21049052) »