Recombinant Human Prostaglandin D Synthase (Lipocalin) protein (ab125636)

Overview

Description

  • Nature
    Recombinant
  • Source
    Escherichia coli
  • Amino Acid Sequence
    • Accession
    • Species
      Human
    • Sequence
      MGSSHHHHHH SSGLVPRGSH MAPEAQVSVQ PNFQQDKFLG RWFSAGLASN SSWLREKKAA LSMCKSVVAP ATDGGLNLTS TFLRKNQCET RTMLLQPAGS LGSYSYRSPH WGSTYSVSVV ETDYDQYALL YSQGSKGPGE DFRMATLYSR TQTPRAELKE KFTAFCKAQG FTEDTIVFLP QTDKCMTEQ
    • Molecular weight
      21 kDa including tags
    • Amino acids
      23 to 190
    • Tags
      His tag N-Terminus

Specifications

Our Abpromise guarantee covers the use of ab125636 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    SDS-PAGE

  • Purity
    > 90 % SDS-PAGE.
    ab125636 is purified by proprietary chromatographic techniques.
  • Form
    Liquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

    pH: 8.00
    Constituents: 0.02% DTT, 0.32% Tris HCl, 0.03% EDTA, 30% Glycerol, 0.58% Sodium chloride

General Info

  • Alternative names
    • Beta trace protein
    • Beta-trace protein
    • Cerebrin 28
    • Cerebrin-28
    • glutathione independent PGD synthase
    • Glutathione independent PGD synthetase
    • Glutathione-independent PGD synthase
    • L PGDS
    • Lipocalin type prostaglandin D synthase
    • Lipocalin-type prostaglandin-D synthase
    • LPGDS
    • PDS
    • PGD2
    • PGD2 synthase
    • PGDS
    • PGDS2
    • prostaglandin D synthase
    • Prostaglandin D2 synthase
    • prostaglandin D2 synthase (21 kDa, brain)
    • prostaglandin D2 synthase (brain)
    • prostaglandin D2 synthase 21kDa (brain)
    • prostaglandin H2 D isomerase
    • Prostaglandin-D2 synthase
    • Prostaglandin-H2 D-isomerase
    • PTGDS
    • PTGDS_HUMAN
    • RP23-47P18.11-006
    see all
  • Function
    Catalyzes the conversion of PGH2 to PGD2, a prostaglandin involved in smooth muscle contraction/relaxation and a potent inhibitor of platelet aggregation. Involved in a variety of CNS functions, such as sedation, NREM sleep and PGE2-induced allodynia, and may have an anti-apoptotic role in oligodendrocytes. Binds small non-substrate lipophilic molecules, including biliverdin, bilirubin, retinal, retinoic acid and thyroid hormone, and may act as a scavenger for harmful hydrophopic molecules and as a secretory retinoid and thyroid hormone transporter. Possibly involved in development and maintenance of the blood-brain, blood-retina, blood-aqueous humor and blood-testis barrier. It is likely to play important roles in both maturation and maintenance of the central nervous system and male reproductive system.
  • Tissue specificity
    Abundant in the brain and CNS, where it is expressed in tissues of the blood-brain barrier and secreted into the cerebro-spinal fluid. Abundantly expressed in the heart. In the male reproductive system, it is expressed in the testis, epididymis and prostate, and is secreted into the seminal fluid. Expressed in the eye and secreted into the aqueous humor. Lower levels detected in various tissue fluids such as serum, normal urine, ascitic fluid and tear fluid. Also found in a number of other organs including ovary, fimbriae of the fallopian tubes, kidney, leukocytes.
  • Sequence similarities
    Belongs to the calycin superfamily. Lipocalin family.
  • Developmental stage
    Expression in the amniotic fluid increases dramatically during weeks 12 to 25 of pregnancy. Levels decrease slowly after 25 weeks.
  • Domain
    Forms a beta-barrel structure that accommodates hydrophobic ligands in its interior.
  • Post-translational
    modifications
    Both N-glycosylation recognition sites are almost quantitatively occupied by N-glycans of the biantennary complex type, with a considerable proportion of structures bearing a bisecting GlcNAc. Agalacto structure as well as sialylated and nonsialylated oligosaccharides bearing alpha2-3- and/or alpha2-6-linked NeuNAc are present.
  • Cellular localization
    Rough endoplasmic reticulum. Nucleus membrane. Golgi apparatus. Cytoplasm > perinuclear region. Secreted. Detected on rough endoplasmic reticulum of arachnoid and menigioma cells. Localized to the nuclear envelope, Golgi apparatus, secretory vesicles and spherical cytoplasmic structures in arachnoid trabecular cells, and to circular cytoplasmic structures in meningeal macrophages and perivascular microglial cells. In oligodendrocytes, localized to the rough endoplasmic reticulum and nuclear envelope. In retinal pigment epithelial cells, localized to distinct cytoplasmic domains including the perinuclear region. Also secreted.
  • Information by UniProt

References

ab125636 has not yet been referenced specifically in any publications.

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