Recombinant Human Proteasome 20S beta 3 protein (ab117227)

Overview

  • Product name
    Recombinant Human Proteasome 20S beta 3 protein
  • Protein length
    Full length protein

Description

  • Nature
    Recombinant
  • Source
    Escherichia coli
  • Amino Acid Sequence
    • Accession
    • Species
      Human
    • Sequence
      MGSSHHHHHHSSGLVPRGSHMSIMSYNGGAVMAMKGKNCVAIAADRRFGI QAQMVTTDFQKIFPMGDRLYIGLAGLATDVQTVAQRLKFRLNLYELKEGR QIKPYTLMSMVANLLYEKRFGPYYTEPVIAGLDPKTFKPFICSLDLIGCP MVTDDFVVSGTCAEQMYGMCESLWEPNMDPDHLFETISQAMLNAVDRDAV SGMGVIVHIIEKDKITTRTLKARMD
    • Molecular weight
      25 kDa including tags
    • Amino acids
      1 to 246
    • Tags
      His tag N-Terminus

Specifications

Our Abpromise guarantee covers the use of ab117227 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Mass Spectrometry

    SDS-PAGE

  • Mass spectrometry
    MALDI-TOF
  • Purity
    > 90 % SDS-PAGE.
    ab117227 is purified using conventional chromatography techniques.
  • Form
    Liquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

    pH: 8.00
    Constituents: 0.32% Tris HCl, 0.02% DTT, 50% Glycerol, 1.17% Sodium chloride

General Info

  • Alternative names
    • C10 II
    • HC10 II
    • proteasome (prosome, macropain) subunit, beta type 3
    • Proteasome beta 3 subunit
    • Proteasome chain 13
    • proteasome component C10 II
    • Proteasome component C10-II
    • Proteasome subunit beta type-3
    • Proteasome theta chain
    • PSB3_HUMAN
    • PSMB3
    see all
  • Function
    The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity.
  • Sequence similarities
    Belongs to the peptidase T1B family.
  • Cellular localization
    Cytoplasm. Nucleus.
  • Information by UniProt

Images

  • 15% SDS-PAGE showing ab117227 at approximately 25.1kDa (3µg).

References

ab117227 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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