The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Additional notesab116427 is stable 4°C for 4 weeks, should be stored desiccated below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
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Preparation and Storage
Stability and Storage
Shipped at 4°C. Please see notes section.
pH: 7.40 Constituents: 89% PBS, 10% Glycerol
26S proteasome non-ATPase regulatory subunit 10
26S proteasome regulatory subunit p28
Ankyrin repeat protein
Hepatocellular carcinoma-associated protein p28 II
Proteasome (prosome, macropain) 26S subunit, non ATPase, 10
FunctionActs as a chaperone during the assembly of the 26S proteasome, specifically of the PA700/19S regulatory complex (RC). In the initial step of the base subcomplex assembly is part of an intermediate PSMD10:PSMC4:PSMC5:PAAF1 module which probably assembles with a PSMD5:PSMC2:PSMC1:PSMD2 module. Acts as an proto-oncoprotein by being involved in negative regulation of tumor suppressors RB1 and p53/TP53. Overexpression is leading to phosphorylation of RB1 and proteasomal degradation of RB1. Regulates CDK4-mediated phosphorylation of RB1 by competing with CDKN2A for binding with CDK4. Facilitates binding of MDM2 to p53/TP53 and the mono- and polyubiquitination of p53/TP53 by MDM2 suggesting a function in targeting the TP53:MDM2 complex to the 26S proteasome. Involved in p53-independent apoptosis. Involved in regulation of NF-kappa-B by retaining it in the cytoplasm. Binds to the NF-kappa-B component RELA and accelerates its XPO1/CRM1-mediated nuclear export.
Tissue specificityOverexpressed in hepatocellular carcinomas.