Recombinant Human Proteasome 26S S10 protein (ab78763)

Overview

Description

  • NatureRecombinant
  • SourceEscherichia coli
  • Amino Acid Sequence
    • SpeciesHuman
    • SequenceMEGCVSNLMV CNLAYSGKLE ELKESILADK SLATRTDQDS RTALHWACSA GHTEIVEFLL QLGVPVNDKD DAGWSPLHIA ASAGRDEIVK ALLGKGAQVN AVNQNGCTPL HYAASKNRHE IAVMLLEGGA NPDAKDHYEA TAMHRAAAKG NLKMIHILLY YKASTNIQDT EGNTPLHLAC DEERVEEAKL LVSQGASIYI ENKEEKTPLQ VAKGGLGLIL KRMVEG

Associated products

Specifications

Our Abpromise guarantee covers the use of ab78763 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    SDS-PAGE

  • Purity> 95 % SDS-PAGE.
    ab78763 is purified by using anion-exchange chromatography (DEAE sepharose resin) and gel-filtration chromatography (Sephacryl S-200) with 20mM Tris pH 7.5, 2mM EDTA.
  • FormLiquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

    Preservative: None
    Constituents: 10% Glycerol, PBS, pH 7.4

General Info

  • Alternative names
    • 26S proteasome non-ATPase regulatory subunit 10
    • 26S proteasome regulatory subunit p28
    • Ankyrin repeat protein
    • dJ889N15.2
    • Gankyrin
    • Hepatocellular carcinoma-associated protein p28 II
    • p28
    • p28(GANK)
    • Proteasome (prosome, macropain) 26S subunit, non ATPase, 10
    • PSD10_HUMAN
    • Psmd10
    see all
  • FunctionActs as a chaperone during the assembly of the 26S proteasome, specifically of the PA700/19S regulatory complex (RC). In the initial step of the base subcomplex assembly is part of an intermediate PSMD10:PSMC4:PSMC5:PAAF1 module which probably assembles with a PSMD5:PSMC2:PSMC1:PSMD2 module.
    Acts as an proto-oncoprotein by being involved in negative regulation of tumor suppressors RB1 and p53/TP53. Overexpression is leading to phosphorylation of RB1 and proteasomal degradation of RB1. Regulates CDK4-mediated phosphorylation of RB1 by competing with CDKN2A for binding with CDK4. Facilitates binding of MDM2 to p53/TP53 and the mono- and polyubiquitination of p53/TP53 by MDM2 suggesting a function in targeting the TP53:MDM2 complex to the 26S proteasome. Involved in p53-independent apoptosis. Involved in regulation of NF-kappa-B by retaining it in the cytoplasm. Binds to the NF-kappa-B component RELA and accelerates its XPO1/CRM1-mediated nuclear export.
  • Tissue specificityOverexpressed in hepatocellular carcinomas.
  • Sequence similaritiesContains 7 ANK repeats.
  • Cellular localizationCytoplasm. Nucleus.
  • Information by UniProt

Recombinant Human Proteasome 26S S10 protein images

  • 15% SDS-PAGE showing ab78763 at approximately 24kDa (3µg).

References for Recombinant Human Proteasome 26S S10 protein (ab78763)

ab78763 has not yet been referenced specifically in any publications.

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