Recombinant human Protein Kinase D2 (ab60875)

Overview

Description

  • NatureRecombinant
  • SourceBaculovirus infected Sf9 cells
  • Amino Acid Sequence
    • SpeciesHuman
    • TagsGST tag N-Terminus

Specifications

Our Abpromise guarantee covers the use of ab60875 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    SDS-PAGE

    Functional Studies

  • FormLiquid
  • Additional notes

    ab204856 (CREB peptide) can be utilized as a substrate for assessing kinase activity

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    Preservative: None
    Constituents: 25% Glycerol, 50mM Tris HCl, 150mM Sodium chloride, 0.25mM DTT, 0.1mM EGTA, 0.1mM EDTA, 0.1mM PMSF, pH 7.5

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

General Info

  • Alternative names
    • DKFZp586E0820
    • HSPC187
    • KPCD2_HUMAN
    • nPKC D2
    • nPKC-D2
    • PKD2
    • PRKD 2
    • Prkd2
    • Protein kinase D2
    • Serine/threonine protein kinase D2
    • Serine/threonine-protein kinase D2
    see all
  • FunctionConverts transient diacylglycerol (DAG) signals into prolonged physiological effects, downstream of PKC. Involved in resistance to oxidative stress.
  • Tissue specificityWidely expressed.
  • Sequence similaritiesBelongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. PKD subfamily.
    Contains 1 PH domain.
    Contains 2 phorbol-ester/DAG-type zinc fingers.
    Contains 1 protein kinase domain.
  • Post-translational
    modifications
    Phosphorylation of Ser-876 correlates with the activation status of the kinase.
    Ser-706 is probably phosphorylated by PKC.
  • Cellular localizationCytoplasm. Membrane. Translocation to the cell membrane is required for kinase activation.
  • Information by UniProt

Recombinant human Protein Kinase D2 images

  • Sample Kinase Activity Plot.
  • ab60875 on SDS-PAGE, MW ~130 kDa.

References for Recombinant human Protein Kinase D2 (ab60875)

ab60875 has not yet been referenced specifically in any publications.

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