Overview

Description

  • Nature
    Recombinant
  • Source
    Escherichia coli
  • Amino Acid Sequence
    • Accession
    • Species
      Human
    • Sequence
      MGSSHHHHHH SSGLVPRGSH MGSHM MLYAA TRATLKKEFG GGHIKDEVFG TVKEDVSLHG YKKYLLSQSS PAPLTAAEEE LRQIKINEVQ TDVGVDTKHQ TLQGVAFPIS REAFQALEKL NNRQLNYVQL EIDIKNEIII LANTTNTELK DLPKRIPKDS ARYHFFLYKH SHEGDYLESI VFIYSMPGYT CSIRERMLYS SCKSRLLEIV ERQLQMDVIR KIEIDNGDEL TADFLYEEVH PKQHAHKQSF AKPKGPAGKR GIRRLIRGPA ETEATTD
    • Molecular weight
      32 kDa including tags
    • Amino acids
      100 to 350
    • Tags
      His tag N-Terminus

Specifications

Our Abpromise guarantee covers the use of ab116148 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    SDS-PAGE

  • Mass spectrometry
    MALDI-TOF
  • Purity
    > 80 % SDS-PAGE.
    purified by using conventional chromatography
  • Form
    Liquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

    pH: 8.00
    Constituents: 0.32% Tris HCl, 40% Glycerol, 0.88% Sodium chloride, 0.02% DTT

General Info

  • Alternative names
    • A6
    • A6 protein tyrosine kinase
    • MGC105817
    • MGC23788
    • MGC41876
    • Protein A6
    • Protein tyrosine kinase 9
    • PTK9
    • PTK9 protein tyrosine kinase 9
    • TWF 1
    • twf1
    • TWF1_HUMAN
    • Twinfilin
    • Twinfilin 1
    • Twinfilin actin binding protein, homolog 1
    • Twinfilin, actin-binding protein, homolog 1 (Drosophila)
    • Twinfilin, Drosophila, homolog of, 1
    • Twinfilin-1
    see all
  • Function
    Actin-binding protein involved in motile and morphological processes. Inhibits actin polymerization, likely by sequestering G-actin. By capping the barbed ends of filaments, it also regulates motility. Seems to play an important role in clathrin-mediated endocytosis and distribution of endocytic organelles.
  • Tissue specificity
    Expressed at high levels in the colon, testis, ovary, prostate and lung. Expressed at lower levels in the brain, bladder and heart. Not detected in liver.
  • Sequence similarities
    Belongs to the actin-binding proteins ADF family. Twinfilin subfamily.
    Contains 2 ADF-H domains.
  • Post-translational
    modifications
    Phosphorylated on serine and threonine residues.
  • Cellular localization
    Cytoplasm. Cytoplasm > cytoskeleton. Diffuse cytoplasmic localization with perinuclear and G-actin-rich cortical actin structures sublocalization. Also found at membrane ruffles and cell-cell contacts.
  • Information by UniProt

Images

  • 15% SDS-PAGE showing ab116148 at approximately 31.5kDa (3µg)

References

ab116148 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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