The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Biological activitySpecific activity: 6,000-12,000units/mg. Enzymatic activity was confirmed by measuring the amount of enzyme hydrolyzing 1 nmole of p-nitrophenyl phosphate (pNPP) per minute at 37°C, pH7.5 using 10mM of substrate. Activity Assay:
Prepare a 200ul reaction mix into a suitable container: The final concentrations are 10mM Tris(pH7.5), 50mM NaCl, 2mM DTT, 1mM MnCl2, 10mM pNPP.
Pre-warm reaction mix in heat block at 37°C
Add recombinant PTP1B solution with various concentrations (2ug, 3ug, 4ug) in 200ul reaction buffer.
Mix by inversion and Incubate at 37°C for 10minutes.
Stop the reaction by adding the 800ul of 0.1N NaOH.
Record the absorbance at 405nm
% SDS-PAGE. This protein was purified by conventional column chromatographic techniques
Concentration information loading...
Preparation and Storage
Stability and Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Non receptor tyrosine phosphatase 1
Protein phosphotyrosylphosphatase 1B
Protein tyrosine phosphatase 1B
Protein tyrosine phosphatase non receptor type 1
Protein tyrosine phosphatase placental
Protein-tyrosine phosphatase 1B
Tyrosine protein phosphatase non receptor type 1
Tyrosine-protein phosphatase non-receptor type 1
FunctionMay play an important role in CKII- and p60c-src-induced signal transduction cascades.
Sequence similaritiesBelongs to the protein-tyrosine phosphatase family. Non-receptor class 1 subfamily. Contains 1 tyrosine-protein phosphatase domain.
Post-translational modificationsOxidized on Cys-215; the Cys-SOH formed in response to redox signaling reacts with the alpha-amido of the following residue to form a 4-amino-3-isothiazolidinone serine cross-link, triggering a conformational change that inhibits substrate binding and activity. The active site can be restored by reduction.