Overview

Description

  • NatureRecombinant
  • SourceEscherichia coli
  • Amino Acid Sequence
    • SpeciesHuman
    • SequenceMEMEKEFEQI DKSGSWAAIY QDIRHEASDF PCRVAKLPKN KNRNRYRDVS PFDHSRIKLH QEDNDYINAS LIKMEEAQRS YILTQGPLPN TCGHFWEMVW EQKSRGVVML NRVMEKGSLK CAQYWPQKEE KEMIFEDTNL KLTLISEDIK SYYTVRQLEL ENLTTQETRE ILHFHYTTWP DFGVPESPAS FLNFLFKVRE SGSLSPEHGP VVVHCSAGIG RSGTFCLADT CLLLMDKRKD PSSVDIKKVL LEMRKFRMGL IQTADQLRFS YLAVIEGAKF IMGDSSVQDQ WKELSHEDLE PPPEHIPPPP RPPKRILEPH N
    • Amino acids1 to 321

Specifications

Our Abpromise guarantee covers the use of ab51277 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Biological activitySpecific activity: 6,000-12,000units/mg. Enzymatic activity was confirmed by measuring the amount of enzyme hydrolyzing 1 nmole of p-nitrophenyl phosphate (pNPP) per minute at 37°C, pH7.5 using 10mM of substrate.
    Activity Assay:
    1. Prepare a 200ul reaction mix into a suitable container: The final concentrations are 10mM Tris(pH7.5), 50mM NaCl, 2mM DTT, 1mM MnCl2, 10mM pNPP.
    2. Pre-warm reaction mix in heat block at 37°C
    3. Add recombinant PTP1B solution with various concentrations (2ug, 3ug, 4ug) in 200ul reaction buffer.
    4. Mix by inversion and Incubate at 37°C for 10minutes.
    5. Stop the reaction by adding the 800ul of 0.1N NaOH.
    6. Record the absorbance at 405nm
  • Applications

    SDS-PAGE

    Functional Studies

  • Purity> 95 % SDS-PAGE.
    This protein was purified by conventional column chromatographic techniques
  • FormLiquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

    Preservative: None
    Constituents: 20% Glycerol, 25mM Tris HCl, 2mM Beta mercaptoethanol, 1mM EDTA, 1mM DTT, pH 7.5

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

General Info

  • Alternative names
    • PTP1B
    • Non receptor tyrosine phosphatase 1
    • Protein phosphotyrosylphosphatase 1B
    • Protein tyrosine phosphatase 1B
    • Protein tyrosine phosphatase non receptor type 1
    • Protein tyrosine phosphatase placental
    • Protein-tyrosine phosphatase 1B
    • PTN1_HUMAN
    • PTP 1B
    • PTP-1B
    • PTPN 1
    • PTPN1
    • Tyrosine protein phosphatase non receptor type 1
    • Tyrosine-protein phosphatase non-receptor type 1
    see all
  • FunctionMay play an important role in CKII- and p60c-src-induced signal transduction cascades.
  • Sequence similaritiesBelongs to the protein-tyrosine phosphatase family. Non-receptor class 1 subfamily.
    Contains 1 tyrosine-protein phosphatase domain.
  • Post-translational
    modifications
    Oxidized on Cys-215; the Cys-SOH formed in response to redox signaling reacts with the alpha-amido of the following residue to form a 4-amino-3-isothiazolidinone serine cross-link, triggering a conformational change that inhibits substrate binding and activity. The active site can be restored by reduction.
  • Cellular localizationEndoplasmic reticulum membrane.
  • Information by UniProt

Recombinant human PTP1B protein images

  • 12% SDS PAGE Gel loaded with 3 ug

References for Recombinant human PTP1B protein (ab51277)

ab51277 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"