The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Biological activitySpecific Activity: 1 U/µg. One unit will hydrolyze 1 nmol p-nitrophenyl phosphate per minute at pH 7.4 and 30°C. Assay buffer: 50 mM HEPES, pH 7.4, 2 mM EDTA, 3mM DTT, 100 mM NaCl, 50 mM pNPP.
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Preparation and Storage
Stability and Storage
Shipped on Dry Ice. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.