Recombinant human PKM2 protein (ab196081)
Key features and details
- Expression system: Escherichia coli
- Purity: >= 80% SDS-PAGE
- Active: Yes
- Tags: His tag N-Terminus
- Suitable for: SDS-PAGE, Functional Studies
Description
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Product name
Recombinant human PKM2 protein
See all PKM2 proteins and peptides -
Biological activity
Specific Activity: ≥54 pmol/min/µg
Assay Conditions: A 50 µl reaction is conducted in a buffer containing 50 mM Tris (pH 7.4), 10 mM MgCl2, 50 mM KCl, 2 mM ADP, 10 mM phosphoenolpyruvate (PEP) at room temperature for 15 min. ATP production is detected using Kinase Assay. -
Purity
>= 80 % SDS-PAGE. -
Expression system
Escherichia coli -
Accession
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Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Sequence
MHHHHHHSKPHSEAGTAFIQTQQLHAAMADTFLEHMCRLDIDSPPITARN TGIICTIGPASRSVETLKEMIKSGMNVARLNFSHGTHEYHAETIKNVRTA TESFASDPILYRPVAVALDTKGPEIRTGLIKGSGTAEVELKKGATLKITL DNAYMEKCDENILWLDYKNICKVVEVGSKIYVDDGLISLQVKQKGADFLV TEVENGGSLGSKKGVNLPGAAVDLPAVSEKDIQDLKFGVEQDVDMVFASF IRKASDVHEVRKVLGEKGKNIKIISKIENHEGVRRFDEILEASDGIMVAR GDLGIEIPAEKVFLAQKMMIGRCNRAGKPVICATQMLESMIKKPRPTRAE GSDVANAVLDGADCIMLSGETAKGDYPLEAVRMQHLIAREAEAAMFHRKL FEELVRASSHSTDLMEAMAMGSVEASYKCLAAALIVLTESGRSAHQVARY RPRAPIIAVTRNPQTARQAHLYRGIFPVLCKDPVQEAWAEDVDLRVNFAM NVGKARGFFKKGDVVIVLTGWRPGSGFTNTMRVVPVP -
Predicted molecular weight
59 kDa including tags -
Amino acids
1 to 531 -
Tags
His tag N-Terminus -
Additional sequence information
GenBank Accession No. NM_182470
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Associated products
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Related Products
Specifications
Our Abpromise guarantee covers the use of ab196081 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
SDS-PAGE
Functional Studies
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Form
Liquid -
Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped on Dry Ice. Store at -80°C. Avoid freeze / thaw cycle.
pH: 8.00
Preservative: 0.45% Imidazole
Constituents: 0.63% Tris HCl, 0.64% Sodium chloride, 0.02% Potassium chloride, 20% Glycerol (glycerin, glycerine), 0.05% (R*,R*)-1,4-Dimercaptobutan-2,3-diolThis product is an active protein and may elicit a biological response in vivo, handle with caution.
General Info
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Alternative names
- CTHBP
- Cytosolic thyroid hormone binding protein
- Cytosolic thyroid hormone-binding protein
see all -
Function
Glycolytic enzyme that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate (PEP) to ADP, generating ATP. Stimulates POU5F1-mediated transcriptional activation. Plays a general role in caspase independent cell death of tumor cells. The ratio between the highly active tetrameric form and nearly inactive dimeric form determines whether glucose carbons are channeled to biosynthetic processes or used for glycolytic ATP production. The transition between the 2 forms contributes to the control of glycolysis and is important for tumor cell proliferation and survival. -
Tissue specificity
Specifically expressed in proliferating cells, such as embryonic stem cells, embryonic carcinoma cells, as well as cancer cells. -
Pathway
Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 5/5. -
Sequence similarities
Belongs to the pyruvate kinase family. -
Post-translational
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR.
ISGylated. -
Cellular localization
Cytoplasm. Nucleus. Translocates to the nucleus in response to different apoptotic stimuli. Nuclear translocation is sufficient to induce cell death that is caspase independent, isoform-specific and independent of its enzymatic actvity. - Information by UniProt
Images
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab196081 has not yet been referenced specifically in any publications.