The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
This protein has been shown to stimulate protein synthesis in L6 myoblasts at an ED50 of ≤ 10 ng/ml.
R3 IGFI is a recombinant analog of insulin-like growth factor containing the complete human IGFI amino acid sequence with substitution of Arg for Glu 3. It was developed as an inexpensive, high quality potent analog of IGF for use as a growth factor supplement for serum-free or low serum cell culture. Recombinantly produced in E. coli using a patented expression system, the peptide is harvested from cells in inclusion bodies, which are dissolved and desalted. The active molecule is properly folded under oxidizing conditions and then purified by several liquid chromatography steps.
NOTE: This protein is full length, SwissProt ID P05019. The protein undergoes molecular processing and the IGF1 is full length at aa 49-118. The rest of the sequence is signal peptide and propeptide
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Preparation and Storage
Stability and Storage
Shipped at 4°C. Store at 4°C prior to reconstitution. Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
The product is lyophilized from 0.1 M acetic acid
This product is an active protein and may elicit a biological response in vivo, handle with caution.
ReconstitutionDissolve in 100 µl of 10 mM HCl to create a 1mg/ml stock. For concentrations of <1 mg/ml in buffer, a carrier protein should be added to minimize adsorption of the peptide to plastic or glass surfaces. BSA between 1–5 mg/ml is recommended in serum-free conditions. A carrier protein not necessary in serum-supplemented media. Up to 400 ml of buffer soln containing BSA or serum-supplemented medium may be added to the stock soln in the vial and mixed. Long-term storage of <1 mg/ml not recommended.
Insulin-like growth factor I
Mechano growth factor
RelevanceR3 IGF1 is an 83 amino acid analog of IGF1 comprising the complete human IGF1 sequence with the substition of an Arg(R) for the Glu(E) at position three, hence R3, and a 13 amino acid extension peptide at the N terminus. This analog of IGF1 has been produced with the purpose of increasing the biological activity of the IGF peptide.
Insulin-like growth factors (IGFs), comprise a family of peptides that play important roles in mammalian growth and development. IGF1 mediates many of the growth-promoting effects of growth hormone.
R3 IGF1 is significantly more potent than IGF1. The enhanced potency is due to the decreased binding of Long R3 IGF1 to all known IGF binding proteins. These binding proteins normally inhibit the biological actions of IGF’s.
References for Recombinant human R3 IGF1 protein (ab58738)
has not yet been referenced specifically in any publications.
Publishing research using ab58738? Please let us know so that we can cite the reference in this datasheet.
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